ADVANCES IN THE USE OF BACILLUS-SUBTILIS FOR THE EXPRESSION AND SECRETION OF HETEROLOGOUS PROTEINS

被引：109

作者：

WONG, SL

机构：

[1] Department of Biological Sciences, Division of Cellular, Molecular and Microbial Biology, University of Calgary, Calgary, Alta. T2N 1N4

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 1995年 / 6卷 / 05期

关键词：

D O I：

10.1016/0958-1669(95)80085-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

During the past year, significant progress has been made using Bacillus subtilis to produce a wide range of foreign proteins. Through strain improvement and co-expression of molecular chaperones, secretory proteins can be produced at a higher level. Through protein engineering, target proteins can be redesigned to have better stability and solubility. A combination of these two strategies would be a useful approach to produce heterologous proteins from B. subtilis at high quality and with a high yield.

引用

页码：517 / 522

页数：6

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