EFFECTS OF ANGIOTENSIN I-CONVERTING ENZYME-INHIBITORY SUBSTANCES DERIVED FROM INDONESIAN DRIED-SALTED FISH ON BLOOD-PRESSURE OF RATS

被引：58

作者：

ASTAWAN, M ^{[1
]}

WAHYUNI, M ^{[1
]}

YASUHARA, T ^{[1
]}

YAMADA, K ^{[1
]}

TADOKORO, T ^{[1
]}

MAEKAWA, A ^{[1
]}

机构：

[1] TOKYO UNIV AGR, JR COLL, DEPT NUTR, SETAGAYA KU, TOKYO 156, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1995年 / 59卷 / 03期

关键词：

D O I：

10.1271/bbb.59.425

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Indonesian dried-salted fish (DSF) was produced from skipjack tuna by soaking the flesh in 15% NaCl (DSF I) or 25% NaCl (DSF II). The DSFs were then hydrolyzed by trypsin, chymotrypsin, Pronase E, and pepsin. Angiotensin I-converting enzyme (ACE) inhibitory activity was measured. The pepsin digest showed the highest inhibitory activity (IC50; 0.63 mg protein/ml). DSF II hydrolysate had higher inhibitory activity than that in DSF I. A three-month storage period of DSF gave higher ACE-inhibitory activity than that of 6 months. An oral administration of pepsin hydrolysate significantly decreased the blood pressure of rats. From the purification steps, at least 4 inhibitor peptides were found. The amino acid sequences of the peptides were Val-Ala-Trp-Lys-Leu, Trp-Ser-Lys-Val-Val-Leu, Ser-Lys-Val-Pro-Pro, and Cys-Trp-Leu-Pro-Val-Tyr, with an IC50 value of 31.97, 156.28, 74.22, and 22.20 mu M, respectively.

引用

页码：425 / 429

页数：5

共 40 条

[1] CHANGES IN PROTEIN-NUTRITIONAL QUALITY OF INDONESIAN DRIED-SALTED FISH AFTER STORAGE [J].

ASTAWAN, M ;

WAHYUNI, M ;

YAMADA, K ;

TADOKORO, T ;

MAEKAWA, A .

JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 1994, 66 (02) :155-161

[2]

CHEUNG HS, 1980, J BIOL CHEM, V255, P401

[3] INHIBITION O HOMOGENEOUS ANGIOTENSIN-CONVERTING ENZYME OF RABBIT LUNG BY SYNTHETIC VENOM PEPTIDES OF BOTHROPS-JARARACA [J].

CHEUNG, HS ;

CUSHMAN, DW .

BIOCHIMICA ET BIOPHYSICA ACTA, 1973, 293 (02) :451-463

[4] INHIBITION OF ANGIOTENSIN-CONVERTING ENZYME BY ANALOGS OF PEPTIDES FROM BOTHROPS-JARARACA VENOM [J].

CUSHMAN, DW ;

PLUSCEC, J ;

WILLIAMS, NJ ;

WEAVER, ER ;

SABO, EF ;

KOCY, O ;

CHEUNG, HS ;

ONDETTI, MA .

EXPERIENTIA, 1973, 29 (08) :1032-1035

[5] ISOLATION OF BRADYKININ-POTENTIATING PEPTIDES FROM BOTHROPS-JARARACA VENOM [J].

FERREIRA, SH ;

BARTELT, DC ;

GREENE, LJ .

BIOCHEMISTRY, 1970, 9 (13) :2583-&

[6]

FOLCH J, 1957, J BIOL CHEM, V226, P497

[7]

GREGER JL, 1993, P SOC EXP BIOL MED, V203, P377, DOI 10.3181/00379727-203-43615

[8] BRADYKININ-POTENTIATING PEPTIDES FROM VENOM OF AGKISTRODON-HALYS-BLOMHOFFII - ISOLATION OF 5 BRADYKININ POTENTIATORS AND AMINO ACID SEQUENCES OF 2 OF THEM, POTENTIATORS-B AND C [J].

KATO, H ;

SUZUKI, T .

BIOCHEMISTRY, 1971, 10 (06) :972-&

[9]

Kawakami A., 1993, Journal of Japanese Society of Nutrition and Food Science [Nihon Eiyo Shokuryo Gakkai-shi], V46, P425

[10] PURIFICATION AND IDENTIFICATION OF AN ANGIOTENSIN I-CONVERTING ENZYME-INHIBITOR FROM SOY-SAUCE [J].

KINOSHITA, E ;

YAMAKOSHI, J ;

KIKUCHI, M .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1993, 57 (07) :1107-1110

← 1 2 3 4 →