COMPETITIVE-PROTEIN-BINDING ASSAY FOR 25-HYDROXYVITAMIN-D

被引：16

作者：

PETTIFOR, JM

ROSS, FP

WANG, J

机构：

[1] UNIV WITWATERSRAND, DEPT PEDIAT, METAB & NUTR RES UNIT, JOHANNESBURG 2001, SOUTH AFRICA

[2] UNIV WITWATERSRAND, DEPT PHYSIOL CHEM, JOHANNESBURG 2001, SOUTH AFRICA

来源：

CLINICAL SCIENCE AND MOLECULAR MEDICINE | 1976年 / 51卷 / 06期

关键词：

D O I：

10.1042/cs0510605

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A modified competitive protein-binding assay for 25-hydroxyvitamin D is described, which does not require the prior separation of vitamin D from 25-hydroxyvitamin D. Bovine albumin was used in the buffer, at a concentration of 1 mg/ml, as a protein stabilizer and lipid solubilizer. Bovine albumin from different manufacturers produced very different nonspecific binding of 25-[3H]hydroxycholecalciferol (ranging from 3.3%-58.9%). The mean concentration of 25-hydroxyvitamin D in sera from normal children in Johannesburg was 76.75 .+-. 24.75 .mu.mol/ml (30.7 .+-. 9.9 ng/ml).

引用

页码：605 / 607

页数：3

共 7 条

[1] COMPETITIVE BINDING ASSAY FOR VITAMIN-D AND 25-OH VITAMIN-D [J].