END-TO-END DIFFUSION OF A FLEXIBLE BICHROMOPHORIC MOLECULE OBSERVED BY INTRAMOLECULAR ENERGY-TRANSFER AND FREQUENCY-DOMAIN FLUOROMETRY

被引：29

作者：

LAKOWICZ, JR ^{[1
]}

KUSBA, J ^{[1
]}

WICZK, W ^{[1
]}

GRYCZYNSKI, I ^{[1
]}

JOHNSON, ML ^{[1
]}

机构：

[1] UNIV VIRGINIA,DEPT PHARMACOL,CHARLOTTESVILLE,VA 22908

来源：

CHEMICAL PHYSICS LETTERS | 1990年 / 173卷 / 04期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1016/0009-2614(90)85277-J

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

We report the first measurement of an end-to-end diffusion coefficient for a flexible molecule using frequency-domain fluorometry. The naphthalene donor was linked to a dansyl acceptor by a polymethylene chain. Frequency-domain intensity decay data were measured for this flexible donor-acceptor pair, in two solvents of modest (48 cP) and low (0.6 cP) viscosity. The data were fit using least-squares criteria with the predicted frequency response for model which includes end-to-end diffusion. Both the initial distance distribution and the diffusion coefficient could be recovered from the data. The resolution of all parameters was improved by global analysis in the low and higher viscosity solvents. © 1990.

引用

页码：319 / 326

页数：8

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