INHIBITORY EFFECT OF ISLET AMYLOID POLYPEPTIDE OF GLUCOSE-INDUCED PROINSULIN BIOSYNTHESIS IN RAT INSULINOMA CELLS

被引：12

作者：

CHUANG, LM

WU, HP

JOU, TS

TAI, TY

LIN, BJI

机构：

[1] Department of Internal Medicine, College of Medicine, National Taiwan University, Taipei, No. 1, Chan-Te St

[2] Graduate Institute of Clinical Medicine, College of Medicine, National Taiwan University, Taipei, No. 1, Chan-Te St

来源：

PANCREAS | 1992年 / 7卷 / 04期

关键词：

AMYLIN; DIABETES-ASSOCIATED PEPTIDE; ISLET AMYLOID POLYPEPTIDE; PROINSULIN BIOSYNTHESIS; RAT INSULINOMA CELL LINE;

D O I：

10.1097/00006676-199207000-00009

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Islet amyloid polypeptide (IAPP) has been recently identified as the principal constituent of amyloid deposits in pancreatic islets of patients with type 2 (non-insulin-dependent) diabetes mellitus and causes insulin resistance in some target cells. In addition, glucose-induced insulin secretion is inhibited by IAPP. We studied the effect of IAPP on proinsulin biosynthesis in rat insulinoma (RINr) cells. Glucose at concentrations of 0, 15, 30, 60, 100, and 300 mg/dl stimulated proinsulin biosynthesis in a dose-responsive and actinomycin D-inhibitable manner after 6 h of incubation. At a glucose concentration of 300 mg/dl, IAPP decreased the mean responses of proinsulin biosynthesis to 61.2 and 29% at concentrations of 0.1 and 1-mu-M, respectively, compared with the IAPP-free control. In conclusion, IAPP inhibits glucose-induced proinsulin biosynthesis in RINr cells. IAPP might play an important role in the pathogenesis of type 2 diabetes mellitus.

引用

页码：472 / 476

页数：5

共 42 条

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