THERMALLY ASSISTED ELECTROSPRAY INTERFACE FOR LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY

被引：28

作者：

LEE, ED ^{[1
]}

HENION, JD ^{[1
]}

机构：

[1] CORNELL UNIV,NEW YORK STATE COLL VET MED,925 WARREN DR,ITHACA,NY 14850

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1992年 / 6卷 / 12期

关键词：

D O I：

10.1002/rcm.1290061204

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An electrospray interface for liquid chromatography/mass spectrometry (LC/MS) is described that combines the direct heating of high-performance liquid chromatography (HPLC) effluent that is characteristic of thermospray-type interfaces. This interface is called the thermally assisted electrospray interface for LC/MS. The device can accept the total effluent from a liquid chromatograph at flow rates ranging up to 500 muL/min. The HPLC effluent is nebulized into charged droplets by a combination of heat and an applied electric field. Ions emitted from charged droplets are mass-analyzed by a triple quadrupole mass spectrometer equipped with an atmospheric pressure ion sampling orifice. Singly or multiply charged ions, indicating the molecular weight of a sample, are observed with minimal fragmentation. The extent of multiple charging observed in electrospray and ion spray mass spectrometry is decreased somewhat in thermally assisted electrospray. Labile compounds which undergo thermal decomposition by conventional thermospray show no decomposition with thermally assisted electrospray.

引用

页码：727 / 733

页数：7

共 25 条

[1] THERMOSPRAY INTERFACE FOR LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY [J].

BLAKLEY, CR ;

VESTAL, ML .

ANALYTICAL CHEMISTRY, 1983, 55 (04) :750-754

[2] DETERMINATION OF SULFONATED AZO DYES BY LIQUID-CHROMATOGRAPHY ATMOSPHERIC-PRESSURE IONIZATION MASS-SPECTROMETRY [J].

BRUINS, AP ;

WEIDOLF, LOG ;

HENION, JD ;

BUDDE, WL .

ANALYTICAL CHEMISTRY, 1987, 59 (22) :2647-2652

[3] ION SPRAY INTERFACE FOR COMBINED LIQUID CHROMATOGRAPHY/ATMOSPHERIC PRESSURE IONIZATION MASS-SPECTROMETRY [J].

BRUINS, AP ;

COVEY, TR ;

HENION, JD .

ANALYTICAL CHEMISTRY, 1987, 59 (22) :2642-2646

[4]

Busch KL., 1988, MASS SPECTROMETRY MA

[5] AN ELECTROSPRAY-IONIZATION MASS-SPECTROMETER WITH NEW FEATURES [J].

CHOWDHURY, SK ;

KATTA, V ;

CHAIT, BT .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1990, 4 (03) :81-87

[6]

Covey T R, 1988, Rapid Commun Mass Spectrom, V2, P249, DOI 10.1002/rcm.1290021111

[7] COMPARISON OF THERMOSPRAY AND ION SPRAY MASS-SPECTROMETRY IN AN ATMOSPHERIC-PRESSURE ION-SOURCE [J].

COVEY, TR ;

BRUINS, AP ;

HENION, JD .

ORGANIC MASS SPECTROMETRY, 1988, 23 (03) :178-186

[8] MOLECULAR BEAMS OF MACROIONS [J].

DOLE, M ;

MACK, LL ;

HINES, RL .

JOURNAL OF CHEMICAL PHYSICS, 1968, 49 (05) :2240-&

[9] ATMOSPHERIC-PRESSURE ION-SAMPLING SYSTEM FOR LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY ANALYSES ON A BENCHTOP MASS-SPECTROMETER [J].

DUFFIN, KL ;

WACHS, T ;

HENION, JD .

ANALYTICAL CHEMISTRY, 1992, 64 (01) :61-68

[10] THE DETERMINATION OF SULFONATED AZO DYES IN MUNICIPAL WASTE-WATER BY ION SPRAY LIQUID-CHROMATOGRAPHY TANDEM MASS-SPECTROMETRY [J].

EDLUND, PO ;

LEE, ED ;

HENION, JD ;

BUDDE, WL .

BIOMEDICAL AND ENVIRONMENTAL MASS SPECTROMETRY, 1989, 18 (04) :233-240

← 1 2 3 →