DETECTION OF A PRFA-INDEPENDENT PROMOTER RESPONSIBLE FOR LISTERIOLYSIN GENE-EXPRESSION IN MUTANT LISTERIA-MONOCYTOGENES STRAINS LACKING THE PRFA REGULATOR

被引：41

作者：

DOMANN, E

WEHLAND, J

NIEBUHR, K

HAFFNER, C

LEIMEISTERWACHTER, M

CHAKRABORTY, T

机构：

[1] UNIV GIESSEN KLINIKUM,INST MED MIKROBIOL,W-6300 GIESSEN,GERMANY

[2] GESELL BIOTECHNOL FORSCH MBH,MIKROBIOL ABT,W-3300 BRAUNSCHWEIG,GERMANY

[3] UNIV WURZBURG,MED KLIN,INST KLIN CHEM & PATHOCHEM,W-8700 WURZBURG,GERMANY

[4] INST VIRION GMBH,W-8700 WURZBURG,GERMANY

来源：

INFECTION AND IMMUNITY | 1993年 / 61卷 / 07期

关键词：

D O I：

10.1128/IAI.61.7.3073-3075.1993

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Expression of listeriolysin, a major virulence factor of pathogenic Listeria monocytogenes, is positively regulated by the pleiotropic virulence regulator PrfA. In this study, we demonstrate that L. monocytogenes strains lacking the prfA regulator gene produce listeriolysin in small, albeit detectable, amounts when analyzed in a hemolysin assay and by immunoblots with listeriolysin-specific monoclonal antibodies. Transcriptional analysis revealed the existence of a PrfA-independent promoter that was responsible for the hemolytic activity expressed by these strains.

引用

页码：3073 / 3075

页数：3

共 14 条

[1] COORDINATE REGULATION OF VIRULENCE GENES IN LISTERIA-MONOCYTOGENES REQUIRES THE PRODUCT OF THE PRFA GENE
CHAKRABORTY, T
LEIMEISTERWACHTER, M
DOMANN, E
HARTL, M
GOEBEL, W
NICHTERLEIN, T
NOTERMANS, S
[J]. JOURNAL OF BACTERIOLOGY, 1992, 174 (02) : 568 - 574
[2] CHAKRABORTY TK, UNPUB
[3] DOMANN E, 1988, NUCLEIC ACIDS RES, V17, P6406
[4] VIRULENCE OF DIFFERENT STRAINS OF LISTERIA-MONOCYTOGENES SEROVAR 1/2A
HOF, H
[J]. MEDICAL MICROBIOLOGY AND IMMUNOLOGY, 1984, 173 (04) : 207 - 218
[5] IDENTIFICATION OF A GENE THAT POSITIVELY REGULATES EXPRESSION OF LISTERIOLYSIN, THE MAJOR VIRULENCE FACTOR OF LISTERIA-MONOCYTOGENES
LEIMEISTERWACHTER, M
HAFFNER, C
DOMANN, E
GOEBEL, W
CHAKRABORTY, T
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (21) : 8336 - 8340
[6] LEIMEISTERWACHTER M, 1989, INFECT IMMUN, V57, P2350
[7] MARSHALL NE, 1991, J IMMUNOL, V147, P2324
[8] PLEIOTROPIC CONTROL OF LISTERIA-MONOCYTOGENES VIRULENCE FACTORS BY A GENE THAT IS AUTOREGULATED
MENGAUD, J
DRAMSI, S
GOUIN, E
VAZQUEZBOLAND, JA
MILON, G
COSSART, P
[J]. MOLECULAR MICROBIOLOGY, 1991, 5 (09) : 2273 - 2283
[9] TRANSCRIPTIONAL MAPPING AND NUCLEOTIDE-SEQUENCE OF THE LISTERIA-MONOCYTOGENES HLYA REGION REVEAL STRUCTURAL FEATURES THAT MAY BE INVOLVED IN REGULATION
MENGAUD, J
VICENTE, MF
COSSART, P
[J]. INFECTION AND IMMUNITY, 1989, 57 (12) : 3695 - 3701
[10] INTRACELLULAR AND CELL-TO-CELL SPREAD OF LISTERIA-MONOCYTOGENES INVOLVES INTERACTION WITH F-ACTIN IN THE ENTEROCYTE-LIKE CELL-LINE CACO-2
MOUNIER, J
RYTER, A
COQUISRONDON, M
SANSONETTI, PJ
[J]. INFECTION AND IMMUNITY, 1990, 58 (04) : 1048 - 1058

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