DIFFERENTIAL RESPONSE OF HUMAN-FIBROBLASTS TO THE CYTO-TOXIC AND MUTAGENIC EFFECTS OF UV-RADIATION IN DIFFERENT PHASES OF THE CELL-CYCLE

被引：25

作者：

ENNINGA, IC ^{[1
]}

GROENENDIJK, RTL ^{[1
]}

VANZEELAND, AA ^{[1
]}

SIMONS, JWIM ^{[1
]}

机构：

[1] INTERUNIV INST RADIOPATHOL & RADIAT PROTECT, JA COHEN INST, LEIDEN, NETHERLANDS

来源：

MUTATION RESEARCH | 1985年 / 148卷 / 1-2期

关键词：

D O I：

10.1016/0027-5107(85)90215-5

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The effects of DNA repair on UV-induced mutagenesis and cell killing in human diploid skin fibroblasts in different phases of the cell cycle were studied. The cells were synchronized in G1 by culturing at 30.degree. C. Using this synchronization method, it could be demonstrated that cells irradiated at 30.degree. C and allowed to carry out excision repair for various lengths of time, show a much lower mutation frequency than cells irradiated in the exponentially growing state. Irradiation in early G1 gives rise to less mutations than irradiation in S. The surviving fraction is not decreased when cells are irradiated in S in comparison with irradiation in G1. There is no recovery from UV-induced lethal effects when irradiated cells are kept stationary at 30.degree. C for various periods of time. This is in contrast with the results obtained with density-inhibited fibroblasts held at 37.degree. C, which show a recovery from the UV-induced lethal effects.

引用

页码：119 / 128

页数：10

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