PROBING STERIC AND HYDROPHOBIC EFFECTS ON ENZYME-SUBSTRATE INTERACTIONS BY PROTEIN ENGINEERING

被引：255

作者：

ESTELL, DA

GRAYCAR, TP

MILLER, JV

POWERS, DB

BURNIER, JP

NG, PG

WELLS, JA

机构：

[1] GENENTECH INC,DEPT BICATALYSIS,460 POINT SAN BRUNO BLVD,SAN FRANCISCO,CA 94080

[2] GENENCOR INC,DEPT RES,SAN FRANCISCO,CA 94080

[3] GENENTECH INC,DEPT DEV BIOL,SAN FRANCISCO,CA 94080

来源：

关键词：

D O I：

10.1126/science.233.4764.659

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

引用

页码：659 / 663

页数：5

共 41 条

[1] BACILLUS-SUBTILIS REQUIRES A STRINGENT SHINE-DALGARNO REGION FOR GENE-EXPRESSION
BAND, L
HENNER, DJ
[J]. DNA-A JOURNAL OF MOLECULAR & CELLULAR BIOLOGY, 1984, 3 (01): : 17 - 21
[2] BOTT R, UNPUB
[3] SITE-DIRECTED MUTAGENESIS AND THE ROLE OF THE OXYANION HOLE IN SUBTILISIN
BRYAN, P
PANTOLIANO, MW
QUILL, SG
HSIAO, HY
POULOS, T
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (11) : 3743 - 3745
[4] SUPERCOIL SEQUENCING - A FAST AND SIMPLE METHOD FOR SEQUENCING PLASMID DNA
CHEN, EY
SEEBURG, PH
[J]. DNA-A JOURNAL OF MOLECULAR & CELLULAR BIOLOGY, 1985, 4 (02): : 165 - 170
[5] CHOTHIA C, 1984, ANNU REV BIOCHEM, V53, P537
[6] REDESIGNING TRYPSIN - ALTERATION OF SUBSTRATE-SPECIFICITY
CRAIK, CS
LARGMAN, C
FLETCHER, T
ROCZNIAK, S
BARR, PJ
FLETTERICK, R
RUTTER, WJ
[J]. SCIENCE, 1985, 228 (4697) : 291 - 297
[7] 3-DIMENSIONAL STRUCTURE AND ITS COMPARISON WITH SUBTILISIN BPN
DRENTH, J
HOL, WGJ
JANSONIUS, JN
KOEKOEK, R
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1972, 26 (02): : 177 - +
[8] ESTELL DA, 1985, J BIOL CHEM, V260, P6518
[9] ESTELL DA, UNPUB
[10] FERSHT AR, 1985, ENZYME STRUCTURE MEC, P293