EXPRESSED SEQUENCE TAGS AND CHROMOSOMAL LOCALIZATION OF CDNA CLONES FROM A SUBTRACTED RETINAL-PIGMENT EPITHELIUM LIBRARY

被引：62

作者：

GIESER, L

SWAROOP, A

机构：

[1] UNIV MICHIGAN, KELLOGG EYE CTR 540, DEPT OPHTHALMOL, 1000 WALL ST, ANN ARBOR, MI 48105 USA

[2] UNIV MICHIGAN, KELLOGG EYE CTR, DEPT HUMAN GENET, ANN ARBOR, MI 48105 USA

来源：

GENOMICS | 1992年 / 13卷 / 03期

关键词：

D O I：

10.1016/0888-7543(92)90173-P

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Expressed sequence tags (ESTs) provide useful molecular landmarks for physical mapping and identify the position of an expressed region in the genome. The use of subtracted cDNA libraries enriched for tissue-specific genes as a source of ESTs should reduce the repetitive isolation of constitutively expressed sequences. We report here the sequence tags from the 3′-end region of 58 new directionally cloned cDNAs from a substracted human retinal pigment epithelium (RPE) cell line library. Eight of the cDNAs have been assigned to human chromosomes using PCR-based EST assays. Chromosomal mapping of subtracted RPE cDNA clones may also help in identifying candidate genes for inherited eye diseases. © 1992.

引用

收藏

页码：873 / 876

页数：4

相关论文

共 12 条

[1] COMPLEMENTARY-DNA SEQUENCING - EXPRESSED SEQUENCE TAGS AND HUMAN GENOME PROJECT [J].

ADAMS, MD ;

KELLEY, JM ;

GOCAYNE, JD ;

DUBNICK, M ;

POLYMEROPOULOS, MH ;

XIAO, H ;

MERRIL, CR ;

WU, A ;

OLDE, B ;

MORENO, RF ;

KERLAVAGE, AR ;

MCCOMBIE, WR ;

VENTER, JC .

SCIENCE, 1991, 252 (5013) :1651-1656

[2] ISOLATION OF CDNAS OF SCRAPIE-MODULATED RNAS BY SUBTRACTIVE HYBRIDIZATION OF A CDNA LIBRARY [J].

DUGUID, JR ;

ROHWER, RG ;

SEED, B .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (15) :5738-5742

[3] SYSTEMATIC GENERATION OF SEQUENCE-TAGGED SITES FOR PHYSICAL MAPPING OF HUMAN-CHROMOSOMES - APPLICATION TO THE MAPPING OF HUMAN CHROMOSOME-7 USING YEAST ARTIFICIAL CHROMOSOMES [J].

GREEN, ED ;

MOHR, RM ;

IDOL, JR ;

JONES, M ;

BUCKINGHAM, JM ;

DEAVEN, LL ;

MOYZIS, RK ;

OLSON, MV .

GENOMICS, 1991, 11 (03) :548-564

[4] DETECTION OF SINGLE BASE SUBSTITUTIONS IN TOTAL GENOMIC DNA [J].

MYERS, RM ;

LUMELSKY, N ;

LERMAN, LS ;

MANIATIS, T .

NATURE, 1985, 313 (6002) :495-498

[5] A COMMON LANGUAGE FOR PHYSICAL MAPPING OF THE HUMAN GENOME [J].

OLSON, M ;

HOOD, L ;

CANTOR, C ;

BOTSTEIN, D .

SCIENCE, 1989, 245 (4925) :1434-1435

[6] RAPID AND SENSITIVE DETECTION OF POINT MUTATIONS AND DNA POLYMORPHISMS USING THE POLYMERASE CHAIN-REACTION [J].

ORITA, M ;

SUZUKI, Y ;

SEKIYA, T ;

HAYASHI, K .

GENOMICS, 1989, 5 (04) :874-879

[7] A SIMPLE SUBTRACTIVE HYBRIDIZATION TECHNIQUE EMPLOYING PHOTOACTIVATABLE BIOTIN AND PHENOL EXTRACTION [J].

SIVE, HL ;

STJOHN, T .

NUCLEIC ACIDS RESEARCH, 1988, 16 (22) :10937-10937

[8] CHARON BS(+) AND (-), VERSATILE LAMBDA-PHAGE VECTORS FOR CONSTRUCTING DIRECTIONAL CDNA LIBRARIES AND THEIR EFFICIENT TRANSFER TO PLASMIDS [J].

SWAROOP, A ;

WEISSMAN, SM .

NUCLEIC ACIDS RESEARCH, 1988, 16 (17) :8739-8739

[9] A SIMPLE AND EFFICIENT CDNA LIBRARY SUBTRACTION PROCEDURE - ISOLATION OF HUMAN RETINA-SPECIFIC CDNA CLONES [J].

SWAROOP, A ;

XU, JZ ;

AGARWAL, N ;

WEISSMAN, SM .

NUCLEIC ACIDS RESEARCH, 1991, 19 (08) :1954-1954

[10] Molecular Analysis of the cDNA for Human SPARC/Osteonectin/BM-40: Sequence, Expression, and Localization of the Gene to Chromosome 5q31-q33 [J].

Swaroop, Anand ;

Hogan, Brigid L. M. ;

Francke, Uta .

GENOMICS, 1988, 2 (01) :37-47