MOLECULAR CYTOGENETIC ANALYSIS OF RB-1 DELETIONS IN CHRONIC B-CELL LEUKEMIAS

被引：28

作者：

DOHNER, H

PILZ, T

FISCHER, K

CABOT, G

DIEHL, D

FINK, T

STILGENBAUER, S

BENTZ, M

LICHTER, P

机构：

[1] Medizinisehe Klinik und Poliklinik V., Universität Heidelberg, Heidelberg

[2] Abteilung Organisation Komplexer Genome, Deutsches Krebsforschungszentrum, Heidelberg

来源：

LEUKEMIA & LYMPHOMA | 1994年 / 16卷 / 1-2期

关键词：

B-CELL CHRONIC LYMPHOCYTIC LEUKEMIA; B-PROLYMPHOCYTIC LEUKEMIA; HAIRY CELL LEUKEMIA; FLUORESCENCE IN SITU HYBRIDIZATION; RETINOBLASTOMA TUMOR SUPPRESSOR GENE;

D O I：

10.3109/10428199409114145

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Deletions or translocations of 13q, most commonly involving band 13q14, belong to the most frequent structural chromosome abnormalities in B-cell chronic lymphocytic leukemia (B-CLL). In a combined metaphase and interphase cytogenetic study using conventional G-banding analysis and fluorescence in situ hybridization (ISH) we previously analysed the retinoblastoma susceptibility gene (RB-1) and its chromosomal locus 13q14 in 35 patients with chronic B-cell leukemias. We report here on the interphase cytogenetic analysis of 109 cases with chronic B-cell leukemias [B-CLL = 90; B-prolymphocytic leukemia (B-PLL) = 6, hairy cell leukemia (HCL) = 13]; a subset of 49 patients (B-CLL = 45; B-PLL = 4) was studied by conventional G-banding analysis. By G-bantling, 5/45 (11%) patients with B-CLL had deletions or translocations affecting band 13q14: in contrast, ISH to interphase cells showed RB-1 deletion in 19/90 (21%) patients with B-CLL. No 13q14 abnormalities or RB-1 deletion were detected in patients with B-PLL and HCL. Our data confirm the high frequency of RB-1 deletions in B-CLL and further emphasize the possible pathogenetic role of this genomic region.

引用

页码：97 / 103

页数：7

共 35 条

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