A DETECTION METHOD FOR POINT MUTATION IN THE PRECORE REGION OF HUMAN HEPATITIS-B VIRUS (HBV)-DNA USING MUTATION-SITE-SPECIFIC ASSAY

被引:24
作者
KINOSHITA, M [1 ]
SENO, T [1 ]
FUKUI, T [1 ]
SHIN, S [1 ]
TSUBOTA, A [1 ]
KUMADA, H [1 ]
机构
[1] TORANOMON GEN HOSP,DEPT GASTROENTEROL,KAWASAKI,KANAGAWA 213,JAPAN
关键词
HBV-DNA; POINT MUTATION; MUTATION-SITE-SPECIFIC ASSAY (MSSA); PRECORE REGION;
D O I
10.1016/0009-8981(94)90279-8
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
In the natural progression of acute active hepatitis and chronic active hepatitis in human hepatitis B virus (HBV)-infected patients, inactive hepatitis develops by seroconversion, which can be explained by the disappearance of HBe antigen. However, it has been found that in some patients with hepatitis, alanine aminotransferase levels undergo fluctuation even though their serum is negative for HBe antigen. In these patients, HBV-DNA has been detected in the serum and the HBV-DNA so detected has been considered a cause of worsening liver function. Most HBV-DNA detected in these cases has a point mutation from G to A at the 83rd base in the precore region. As a result of this point mutation, HBV is unable to produce HBe antigen. We have devised a sensitive polymerase chain reaction (PCR) method, a mutation-site-specific assay, for the detection of point mutations at the 83rd base in the precore region using a specific mutation-trapped oligonucleotide primer for the mutant HBV genome.
引用
收藏
页码:83 / 90
页数:8
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