PRODUCTION OF HUMAN TISSUE-TYPE PLASMINOGEN-ACTIVATOR IN DIFFERENT MAMMALIAN-CELL LINES USING AN EPSTEIN-BARR-VIRUS VECTOR

被引：8

作者：

JALANKO, A ^{[1
]}

PIRHONEN, J ^{[1
]}

POHL, G ^{[1
]}

HANSSON, L ^{[1
]}

机构：

[1] KABIGEN AB,STOCKHOLM,SWEDEN

来源：

JOURNAL OF BIOTECHNOLOGY | 1990年 / 15卷 / 1-2期

关键词：

Cell; CHO-K1; HeLa; 293; K-562; Gene expression; Secretion; Spinner flask culture;

D O I：

10.1016/0168-1656(90)90058-J

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Human tissue-type plasminogen activator (t-PA) cDNA inserted into an Epstein-Barr virus (EBV) derived expression vector was transfected into human HeLa, 293, K-562 and hamster CHO-K1 cells and the expression of t-PA was studied. The best t-PA producing cell clones were found among CHO-K1 cells (up to 11 μg d-1 per 106 cells). However, HeLa and 293 cells were most efficiently transfected, e.g. about 70% of the selected cell clones were t-PA positive. The vector DNA copy numbers correlated with the mRNA levels and the protein levels for all cell lines analysed, with the exception for the K-562 cell line, where the production of t-PA was very low. The results obtained indicated that the highest expression levels were achieved in low density cultures. © 1990.

引用

页码：155 / 168

页数：14

共 30 条

[1] THE FIBRIN PLATE METHOD FOR ESTIMATING FIBRINOLYTIC ACTIVITY [J].