SCREENING FOR MICROORGANISMS PRODUCING D-MALATE FROM MALEATE

被引：38

作者：

VANDERWERF, M ^{[1
]}

VANDENTWEEL, WJJ ^{[1
]}

HARTMANS, S ^{[1
]}

机构：

[1] DSM RES & PATENTS,BIOORGAN CHEM SECT,6160 MD GELEEN,NETHERLANDS

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1992年 / 58卷 / 09期

关键词：

D O I：

10.1128/AEM.58.9.2854-2860.1992

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

More than 300 microorganisms were screened for their ability to convert maleate into D-malate as a result of the action of maleate hydratase. Accumulation of fumarate during incubation of permeabilized cells with maleate was shown to be indicative of one of the two enzymes known to transform maleate. The ratio in which fumarate and malate accumulated could be used to estimate the enantiomeric composition of the malate formed. Many strains (n = 128) were found to be capable of converting maleate to D-malate with an enantiomeric purity of more than 97%. Pseudomonas pseudoalcaligenes NCIMB 9867 was selected for more detailed studies. Although this strain was not able to grow on maleate, permeabilized cells were able to degrade maleate to undetectable levels, with a concomitant formation of D-malate. The D-malate was formed with an enantiomeric purity of more than 99.97%.

引用

页码：2854 / 2860

页数：7

共 37 条

[1]

BEHRMAN EJ, 1957, J BIOL CHEM, V228, P923

[2] STUDIES OF THE ENZYME FUMARASE .1. KINETICS AND EQUILIBRIUM [J].

BOCK, RM ;

ALBERTY, RA .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1953, 75 (08) :1921-1925

[3]

BOYER BD, 1990, Patent No. 365212

[4] EQUILIBRIUM-CONSTANTS OF SEVERAL REACTIONS INVOLVED IN THE FERMENTATION OF GLUTAMATE [J].

BUCKEL, W ;

MILLER, SL .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1987, 164 (03) :565-569

[5] MICROBIAL METABOLISM OF PYRIDINE RING - METABOLISM OF 2-HYDROXYPYRIDINES AND 3-HYDROXYPYRIDINES BY MALEAMATE PATHWAY IN ACHROMOBACTER SP [J].

CAIN, RB ;

HOUGHTON, C ;

WRIGHT, KA .

BIOCHEMICAL JOURNAL, 1974, 140 (02) :293-300

[6]

CHIBATA I, 1986, PROGR IND MICROBIOL, V24, P144

[7]

CREMONA T, 1964, J BIOL CHEM, V239, P1457

[8]

DAVIES M, 1956, T FARADAY SOC, V51, P1506

[9]

DERVARTANIAN D. V., 1965, BIOCHIM BIOPHYS ACTA, V105, P424

[10] ISOLATION AND BIOCHEMICAL-CHARACTERIZATION OF MALEIC-ACID HYDRATASE, AN IRON-REQUIRING HYDRO-LYASE [J].

DREYER, JL .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1985, 150 (01) :145-154

← 1 2 3 4 →