FLOW-THROUGH SENSING DEVICE BASED ON DERIVATIVE SYNCHRONOUS FLUORESCENCE MEASUREMENTS FOR THE MULTI-DETERMINATION OF B-6 VITAMERS

被引：23

作者：

CHEN, DH ^{[1
]}

DECASTRO, MDL ^{[1
]}

VALCARCEL, M ^{[1
]}

机构：

[1] WUHAN UNIV,DEPT CHEM,WUHAN,PEOPLES R CHINA

来源：

ANALYTICA CHIMICA ACTA | 1992年 / 261卷 / 1-2期

关键词：

FLUOROMETRY; FLOW SYSTEM; SERUM; VITAMINS;

D O I：

10.1016/0003-2670(92)80201-H

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method for the determination of pyridoxal, pyridoxal-5-phosphate and pyridoxic acid based on derivative synchronous fluorimetric measurements is proposed. The method involves the formation of a fluorescent complex between the analytes and beryllium in ammonia buffers of different pH which make it possible to discriminate between pyridoxal and pyridoxal-5-phosphate as the fluorescent features of the two complexes are similar-in a medium of pH 9.9, but very different at pH 7.9. The calibration range was 0.05-15.00-mu-M (two orders of magnitude lower than in the conventional flow-injection method, and with a relative standard deviation smaller than 4.5% in this range). The method was applied to the analysis of serum samples with good results.

引用

页码：269 / 274

页数：6

共 24 条

[1] FLUOROMETRIC ASSAY OF PYRIDOXAL AND PYRIDOXAL 5'-PHOSPHATE [J].