A SERIES OF VECTORS THAT SIMPLIFY MAMMALIAN GENE TARGETING

被引:4
作者
BROOKES, AJ
STEVENSON, BJ
PORTEOUS, DJ
DORIN, JR
机构
[1] MRC Human Genetics Unit, Western General Hospital, Edinburgh, EH42XU, Crewe Road
关键词
GENE TARGETING; HOMOLOGOUS RECOMBINATION; VECTORS;
D O I
10.1007/BF01977354
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
In order to facilitate the procedure of mammalian gene targeting, we have produced and functionally tested a series of generic vectors. Homologous recombination has been achieved with each vector. The vectors are designed for both replacement and insertional recombination, are suitable for 'hit and run' strategies and contain all necessary genetic elements for both positive-negative and promoterless/gene fusion enrichment of homologous integrations. Multiple unique restriction sites are included to simplify the incorporation of genomic targeting sequences.
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页码:238 / 244
页数:7
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