A SIMPLE STOPPED-FLOW PHOTOMETER

被引:14
作者
HARVEY, RA
机构
[1] Department of Biochemistry, Rutgers Medical School, Rutgers University, New Brunswick
关键词
D O I
10.1016/0003-2697(69)90007-4
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A stopped-flow photometer is described which is simple enough for teaching applications yet sufficiently versatile to serve as a nucleus for a research instrument. The rapid mixing device employs small sample volumes (typically 20 μl of each reactant) and features a mixing time of 4 milliseconds, a pneumatic drive mechanism, and temperature control for the reactants. Rapid reactions can be monitored by changes in transmittance or fluorescence or by measuring simultaneous changes in transmittance and fluorescence. A photometric system with all solidstate electronic components is described along with an oscilloscope triggering circuit. © 1969.
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页码:58 / &
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