MOLECULAR-CLONING AND NUCLEOTIDE-SEQUENCE OF ANOTHER VARIANT OF THE ESCHERICHIA-COLI SHIGA-LIKE TOXIN-II FAMILY

被引:124
作者
GANNON, VPJ [1 ]
TEERLING, C [1 ]
MASRI, SA [1 ]
GYLES, CL [1 ]
机构
[1] UNIV GUELPH,ONTARIO VET COLL,DEPT VET MICROBIOL & IMMUNOL,GUELPH N1G 2W1,ONTARIO,CANADA
来源
JOURNAL OF GENERAL MICROBIOLOGY | 1990年 / 136卷
关键词
D O I
10.1099/00221287-136-6-1125
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Escherichia coli strain H.I.8 (O128:B12) produces low levels of a Shiga-like toxin (SLT) which we have called SLTIIva because of its close relationship with SLTIIv. The Vero cell cytotoxicity of SLTIIva is neutralized by antisera against SLTII and SLTIIv but not by antisera against SLTI. These data indicate that the SLT of strain H.I.8 is a member of the SLTII family. Since SLTIIva shares with SLTIIv the property of having low cytotoxicity to HeLa cells compared with Vero cells, it is appropriate to consider both toxins as variants of SLTII. SLTIIva differs from SLTIIv in that it is more heat-stable. Further, SLTIIv-producing strains of E. coli have only been isolated from pigs while the SLTIIva-producing E. coli strain examined in this study was isolated from a human infant with diarrhoea. The genes for this SLT were cloned from a cosmid library of total cellular DNA by screening recombinants for Vero cell cytotoxicity and with a DNA probe derived from SLTIIv structural genes. Nucleotide sequence analysis was performed on a 2.0 kb AvaII-HincII fragment which encodes the toxin gene. The nucleotide sequence data confirm the close relationship between SLTIIva and SLTIIv: they have 98% nucleotide sequence homology in the B subunit gene and 70.6% homology in the A subunit gene. Comparison of DNA sequences indicated that SLTIIva was most closely related to SLTIIv, closely related to SLTII and less closely related to SLTI.
引用
收藏
页码:1125 / 1135
页数:11
相关论文
共 50 条
[1]  
Ausubel FM., 1995, MOL REPROD DEV, V3rd edn, DOI DOI 10.1002/MRD.1080010210
[2]  
BIRNBOIM HC, 1979, NUCLEIC ACIDS RES, V7, P1513
[3]   VEROTOXIN RECEPTOR GLYCOLIPID IN HUMAN RENAL TISSUE [J].
BOYD, B ;
LINGWOOD, C .
NEPHRON, 1989, 51 (02) :207-210
[4]   A COMPUTER ALGORITHM FOR TESTING POTENTIAL PROKARYOTIC TERMINATORS [J].
BRENDEL, V ;
TRIFONOV, EN .
NUCLEIC ACIDS RESEARCH, 1984, 12 (10) :4411-4427
[5]   HYBRIDIZATION OF ESCHERICHIA-COLI PRODUCING SHIGA-LIKE TOXIN-I, SHIGA-LIKE TOXIN-II, AND A VARIANT OF SHIGA-LIKE TOXIN-II WITH SYNTHETIC OLIGONUCLEOTIDE PROBES [J].
BROWN, JE ;
SETHABUTR, O ;
JACKSON, MP ;
LOLEKHA, S ;
ECHEVERRIA, P .
INFECTION AND IMMUNITY, 1989, 57 (09) :2811-2814
[6]   NUCLEOTIDE-SEQUENCE OF THE SHIGA-LIKE TOXIN GENES OF ESCHERICHIA-COLI [J].
CALDERWOOD, SB ;
AUCLAIR, F ;
DONOHUEROLFE, A ;
KEUSCH, GT ;
MEKALANOS, JJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (13) :4364-4368
[7]  
DEGRANDIS S, 1989, J BIOL CHEM, V264, P12520
[8]   NUCLEOTIDE-SEQUENCE AND PROMOTER MAPPING OF THE ESCHERICHIA-COLI SHIGA-LIKE TOXIN OPERON OF BACTERIOPHAGE-H-19B [J].
DEGRANDIS, S ;
GINSBERG, J ;
TOONE, M ;
CLIMIE, S ;
FRIESEN, J ;
BRUNTON, J .
JOURNAL OF BACTERIOLOGY, 1987, 169 (09) :4313-4319
[9]   PURIFICATION OF AN ESCHERICHIA-COLI SEROGROUP O157-H7 VEROTOXIN AND ITS DETECTION IN NORTH-AMERICAN HEMORRHAGIC COLITIS ISOLATES [J].
DICKIE, N ;
SPEIRS, JI ;
AKHTAR, M ;
JOHNSON, WM ;
SZABO, RA .
JOURNAL OF CLINICAL MICROBIOLOGY, 1989, 27 (09) :1973-1978
[10]  
ENDO Y, 1987, J BIOL CHEM, V262, P5908