EFFICIENT DETERMINATION OF PHYTOECDYSTEROIDS FROM AJUGA SPECIES AND POLYPODIUM-VULGARE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：9

作者：

CAMPS, F

COLL, J

MARCO, MP

TOMAS, J

机构：

[1] Department of Biological Organic Chemistry, CID-CSIC, 083034 Barcelona

来源：

JOURNAL OF CHROMATOGRAPHY | 1990年 / 514卷 / 02期

关键词：

D O I：

10.1016/S0021-9673(01)89391-2

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Efficient chromatographic conditions were established for the simultaneous determination of eight phytoecdysteroids by high-performance liquid chromatography. Spherisorb ODS-2 columns, ultraviolet detection, isopropanol-water as the mobile phase and temperature control were used. Ecdysteroids were obtained by purification of methanol plant extracts with Sep-Pak C18 cartridges. The results compared well with those obtained by other chromatographic methods in terms of resolution, selectivity and efficiency. © 1990.

引用

页码：199 / 207

页数：9

共 15 条

[1] APPLICATION OF COMBINED CHROMATOGRAPHIC TECHNIQUES IN THE SCREENING AND PURIFICATION OF ECDYSTEROIDS [J].