CYCLIC-AMP SYNTHESIS IN ESCHERICHIA-COLI STRAINS BEARING KNOWN DELETIONS IN THE PTS PHOSPHOTRANSFERASE OPERON

被引:85
作者
LEVY, S [1 ]
ZENG, GQ [1 ]
DANCHIN, A [1 ]
机构
[1] INST PASTEUR,UNITE REGULAT EXPRESS GENET,28 RUE DR ROUX,F-75724 PARIS 15,FRANCE
关键词
adenylate cyclase; Phosphoenolpyruvate; reverse genetics;
D O I
10.1016/0378-1119(90)90110-D
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
A series of isogenic strains harboring known deletions in the pts operon of Escherichia coli have been constructed by reverse genetics. Strains bearing deletions for the whole pts operon failed to grow on maltose or on carbon sources of the same class. In these strains the total cAMP synthesis was significantly lower than in a strain deleted only for the crr gene. This indicated that enzyme I or phosphorylated histidine-containing phosphotransferase protein in addition to its role in phosphorylating enzyme IIIGlc, is involved in adenylate cyclase (AC) activation or cAMP excretion. It was further shown that deletions in the pts operon do not affect synthesis of AC. © 1990.
引用
收藏
页码:27 / 33
页数:7
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