RELATIVE ANTIVIRAL ACTIVITY OF INVITRO-SYNTHESIZED MURINE INTERFERON-ALPHA-4 AND ALPHA-1

被引：6

作者：

BEILHARZ, MW ^{[1
]}

SWAMINATHAN, N ^{[1
]}

LAI, CM ^{[1
]}

PITHA, PM ^{[1
]}

BOYER, SJ ^{[1
]}

机构：

[1] JOHNS HOPKINS UNIV,CTR ONCOL,BALTIMORE,MD 21231

来源：

JOURNAL OF INTERFERON RESEARCH | 1991年 / 11卷 / 01期

关键词：

D O I：

10.1089/jir.1991.11.9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Murine interferon-alpha-4 (MuIFN-alpha-4) is notable among the MuIFN-alpha subtypes because it lacks 5 amino acids corresponding to positions 103-107 of the other subtypes, yet is the most highly expressed subtype. (1,2) Site-directed in vitro mutagenesis has been used to modify the genes coding for MuIFN-alpha-4 and MuIFN-alpha-1. The modifications have allowed (i) the in vitro expression of the mature form of each MuIFN-alpha subtype and (ii) the insertion of five amino acids, corresponding to amino acid positions 103-107 of MuIFN-alpha-1, into the MuIFN-alpha-4 sequence. In contrast to previously published data (1,3) MuIFN-alpha-4 and MuIFN-alpha-1 show only a twofold difference in antiviral activity, with MuIFN-alpha-4 being the more active subtype. In keeping with this observation, it was also found that insertion of the five "missing" amino acids into MuIFN-alpha-4 resulted in an analogue MuIFN-alpha with antiviral activity equivalent to MuIFN-alpha-1. It may be inferred from this work that the deletion of amino acids 103-107 (QVGVQ) is solely responsible for the difference in antiviral activity between MuIFN-alpha-4 and MuIFN-alpha-1.

引用

页码：9 / 15

页数：7

共 24 条

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