APPEARANCE OF A NOVEL CA2+ INFLUX PATHWAY IN SF9 INSECT CELLS FOLLOWING EXPRESSION OF THE TRANSIENT RECEPTOR POTENTIAL-LIKE (TRPL) PROTEIN OF DROSOPHILA

被引:92
作者
HU, YF [1 ]
VACA, L [1 ]
ZHU, X [1 ]
BIRNBAUMER, L [1 ]
KUNZE, DL [1 ]
SCHILLING, WP [1 ]
机构
[1] BAYLOR COLL MED,DEPT BIOPHYS & CELL BIOL,HOUSTON,TX 77030
关键词
D O I
10.1006/bbrc.1994.1808
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Activation of phospholipase C, elevation of free cytosolic Ca2+ concentration ([Ca2+](i)) and stimulation of Ca2+ influx have been implicated in Drosophila phototransduction. Electrophysiological studies suggest that trp and trpl proteins may be important for the light-activated Ca2+ current found in Drosophila photoreceptor cells. Although these proteins exhibit homologies to voltage-gated Ca2+ and Na+ channels, their actual function in insect cells and their relation to proteins involved in mammalian cell Ca2+ signaling remains unknown. In the present study, [Ca2+](i) was examined in fura-2-loaded Sf9 insect cells infected with recombinant baculovirus containing cDNA for the trpl protein. Ca2+ influx was examined by use of Ba2+, a Ca2+ surrogate that is not a substrate for Ca2+-pumps or carriers and by measurement of whole-cell membrane currents. The results suggest that expression of trpl is associated with appearance of a Ca2+ permeable, non-selective cation channel formed by the trpl protein. (C) 1994 Academic Inc.
引用
收藏
页码:1050 / 1056
页数:7
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