AGE-DEPENDENT MODULATION OF NEUROPEPTIDE-Y ON ADRENERGIC TRANSMISSION OF GUINEA-PIG VAS-DEFERENS

1. The effect of neuropeptide (NPY) on [H-3]noradrenaline ([H-3]NA) release- and on contractions evoked by field electrical stimulation (FES) was studied in vitro in vas deferens from mature and immature guinea pigs. 2. The evoked tritium overflow (which reflected [H-3]NA release) was determined by liquid scintillation spectrometry. 3. Field electrical stimulation of 5 Hz (trains of 50 pulses in 20 sec intervals) evoked guanethidine-sensitive contractions. 4. NPY (0.01-1-mu-M) dose-dependently inhibited the evoked contractions in both groups of animals. NPY, 1-mu-M, almost completely inhibited the evoked contractions in mature animals, while those in immature guinea pigs were inhibited but only by 80.4 +/- 3.6%. 5. The amount of tritium overflow evoked by 5 Hz stimulation (300 pulses: 15 trains of 20 pulses in 20 sec intervals) was higher in immature guinea pigs (0.46 +/- 0.03%) compared with the amount of the evoked tritium overflow in mature guinea pigs (0.39 +/- 0.02%). 6. NPY, 1-mu-M, inhibited the evoked tritium overflow. The NPY inhibition was more pronounced in vas deferens of mature (45.3 +/- 2.0%) than in immature (25.1 +/- 3.5%) guinea pigs. 7. The results suggest that NPY modulation of adrenergic transmission at the prejunctional level increases with the maturity.