QUANTIFICATION OF POLYCYCLIC AROMATIC-HYDROCARBONS AND NITRO-SUBSTITUTED POLYCYCLIC AROMATIC-HYDROCARBONS AND MUTAGENICITY TESTING FOR THE CHARACTERIZATION OF AMBIENT AIR PARTICULATE MATTER

The polycyclic aromatic hydrocarbon (PAH) mixture isolated from a large sample collected in Philadelphia, PA, was characterized by liquid chromatography (LC), gas chromatography (GC), and gas chromatography-mass spectrometry (GC-MS). A number of the major PAH constituents were quantified by GC and LC. Quantification of the minor constituents was accomplished by further subfractionation of the PAH mixture into eitht fractions based on the number of aromatic carbons in the PAH. These fractions were then characterized by GC and GC-MS. More than 100 PAH components were quantified in this sample. Approximately 40 unsubstituted PAH and 10 methyl-substituted PAH were identified. Several nitro substituted PAH were also found.