A MONOCLONAL ANTIBODY-BASED ENZYME-IMMUNOASSAY FOR HUMAN GMP-140/P-SELECTIN

被引：54

作者：

KATAYAMA, M

HANDA, M

AMBO, H

ARAKI, Y

HIRAI, S

KATO, I

KAWAI, Y

WATANABE, K

IKEDA, Y

机构：

[1] KEIO UNIV HOSP, DEPT LAB MED, SHINJU KU, TOKYO 160, JAPAN

[2] KEIO UNIV HOSP, DEPT INTERNAL MED, CTR BLOOD, SHINJU KU, TOKYO 160, JAPAN

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1992年 / 153卷 / 1-2期

关键词：

GRANULE MEMBRANE PROTEIN-140; MONOCLONAL ANTIBODY; ENZYME IMMUNOASSAY; PLASMA; CD62; P-SELECTIN;

D O I：

10.1016/0022-1759(92)90303-B

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Two hybridoma cell lines producing monoclonal antibodies WGA-1 and PL7-6, reactive only with thrombin-stimulated human platelet have been established. Both these antibodies were investigated for their specific reactivity against GMP-140, based on the amino acid composition analysis of immunopurified antigen and N terminal amino acid sequencing of its protease fragments. A two-site enzyme immunoassay for quantification of human GMP-140 was developed using WGA-1 monoclonal antibody immobilized on 96-well microplates and horseradish peroxidase-labeled PL7-6 monoclonal antibody as detector. The assay was able to measure GMP-140 in serum and plasma with a sensitivity of about 5 ng/ml and a precision better than 10%. This assay will be useful for the detection of GMP-140 derived from platelets or endothelium in biological fluids and tissue extracts.

引用

页码：41 / 48

页数：8

共 18 条

[1] SELECTINS - A FAMILY OF ADHESION RECEPTORS [J].

BEVILACQUA, M ;

BUTCHER, E ;

FURIE, B ;

GALLATIN, M ;

GIMBRONE, M ;

HARLAN, J ;

KISHIMOTO, K ;

LASKY, L ;

MCEVER, R ;

PAULSON, J ;

ROSEN, S ;

SEED, B ;

SIEGELMAN, M ;

SPRINGER, T ;

STOOLMAN, L ;

TEDDER, T ;

VARKI, A ;

WAGNER, D ;

WEISSMAN, I ;

ZIMMERMAN, G .

CELL, 1991, 67 (02) :233-233

[2] ENDOTHELIAL LEUKOCYTE ADHESION MOLECULE-1 - AN INDUCIBLE RECEPTOR FOR NEUTROPHILS RELATED TO COMPLEMENT REGULATORY PROTEINS AND LECTINS [J].

BEVILACQUA, MP ;

STENGELIN, S ;

GIMBRONE, MA ;

SEED, B .

SCIENCE, 1989, 243 (4895) :1160-1165

[3] PLATELET ACTIVATION DURING PREPARATION OF PLATELET CONCENTRATES - A COMPARISON OF THE PLATELET-RICH PLASMA AND THE BUFFY COAT METHODS [J].

FIJNHEER, R ;

PIETERSZ, RNI ;

DEKORTE, D ;

GOUWEROK, CWN ;

DEKKER, WJA ;

REESINK, HW ;

ROOS, D .

TRANSFUSION, 1990, 30 (07) :634-638

[4]

GAMBLE JR, 1990, SCIENCE, V249, P414

[5]

HAMBURGER SA, 1990, BLOOD, V75, P550

[6]

HANDA M, 1986, J BIOL CHEM, V261, P2579

[7]

JOHNSTON GI, 1989, J BIOL CHEM, V264, P1816

[8] CLONING OF GMP-140, A GRANULE MEMBRANE-PROTEIN OF PLATELETS AND ENDOTHELIUM - SEQUENCE SIMILARITY TO PROTEINS INVOLVED IN CELL-ADHESION AND INFLAMMATION [J].

JOHNSTON, GI ;

COOK, RG ;

MCEVER, RP .

CELL, 1989, 56 (06) :1033-1044

[9] PADGEM PROTEIN - A RECEPTOR THAT MEDIATES THE INTERACTION OF ACTIVATED PLATELETS WITH NEUTROPHILS AND MONOCYTES [J].

LARSEN, E ;

CELI, A ;

GILBERT, GE ;

FURIE, BC ;

ERBAN, JK ;

BONFANTI, R ;

WAGNER, DD ;

FURIE, B .

CELL, 1989, 59 (02) :305-312

[10] CLONING OF A LYMPHOCYTE HOMING RECEPTOR REVEALS A LECTIN DOMAIN [J].

LASKY, LA ;

SINGER, MS ;

YEDNOCK, TA ;

DOWBENKO, D ;

FENNIE, C ;

RODRIGUEZ, H ;

NGUYEN, T ;

STACHEL, S ;

ROSEN, SD .

CELL, 1989, 56 (06) :1045-1055

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