PRELIMINARY-ANALYSIS OF CRYSTALS OF 4-OXALOCROTONATE TAUTOMERASE, AN ENZYME COMPOSED OF UNUSUALLY SMALL MONOMERS

被引：3

作者：

DAVENPORT, RC ^{[1
]}

WHITMAN, CP ^{[1
]}

机构：

[1] UNIV TEXAS,COLL PHARM,DIV MEDICINAL CHEM,AUSTIN,TX 78712

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1993年 / 231卷 / 02期

关键词：

CRYSTALS; 4-OXALOCROTONATE TAUTOMERASE; SMALL ENZYMES; BIOREMEDIATION; PSEUDOMONAS-PUTIDA;

D O I：

10.1006/jmbi.1993.1300

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Crystals of 4-oxalocrotonate tautomerase have been obtained by the vapor-diffusion method using polyethylene glycol as precipitant. The crystals belong to the orthorhombic space group P212121, with unit cell parameters a = 118.1, b = 95.6, c = 97.4, α = β = γ = 90° and diffract well to at least 2.7 Å resolution. There are approximately ten 6811 dalton subunits of the enzyme per asymmetric unit, giving a crystal solvent content of 70%. © 1993 Academic Press, Inc.

引用

页码：509 / 512

页数：4

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