A NEW HUMAN GUANYLATE CYCLASE-ACTIVATING PEPTIDE (GCAP-II, UROGUANYLIN) - PRECURSOR CDNA AND COLONIC EXPRESSION

被引：45

作者：

HILL, O

CETIN, Y

CIESLAK, A

MAGERT, HJ

FORSSMANN, WG

机构：

[1] LOWER SAXONY INST PEPTIDE RES,D-30625 HANNOVER,GERMANY

[2] UNIV HANNOVER,SCH MED,DEPT ANAT,D-30625 HANNOVER,GERMANY

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY | 1995年 / 1253卷 / 02期

关键词：

GUANYLIN; GUANYLATE CYCLASE C; GCAP-II; CDNA SEQUENCE; (HUMAN COLON);

D O I：

10.1016/0167-4838(95)00204-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have amplified, cloned, and sequenced 583 bp GCAP-II/uroguanylin-specific cDNA from human colon cDNA first strand. The cDNA codes for a putative 112 amino-acid precursor protein including the sequence of uroguanylin and GCAP-II. Northern blot hybridization revealed a high level expression of the GCAP-II gene in human colon, but not in the kidney. This expression of GCAP-II indicates a pivotal role in cGMP-mediated functions of the colon.

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页码：146 / 149

页数：4

相关论文

共 24 条

[1] MAMMALIAN SUBTILISINS - THE LONG-SOUGHT DIBASIC PROCESSING ENDOPROTEASES [J].

BARR, PJ .

CELL, 1991, 66 (01) :1-3

[2] ACTIVATION OF INTESTINAL CFTR CL- CHANNEL BY HEAT-STABLE ENTEROTOXIN AND GUANYLIN VIA CAMP-DEPENDENT PROTEIN-KINASE [J].

CHAO, AC ;

DESAUVAGE, FJ ;

DONG, YJ ;

WAGNER, JA ;

GOEDDEL, DV ;

GARDNER, P .

EMBO JOURNAL, 1994, 13 (05) :1065-1072

[3] GUANYLIN - AN ENDOGENOUS ACTIVATOR OF INTESTINAL GUANYLATE-CYCLASE [J].

CURRIE, MG ;

FOK, KF ;

KATO, J ;

MOORE, RJ ;

HAMRA, FK ;

DUFFIN, KL ;

SMITH, CE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (03) :947-951

[4] CHLORIDE SECRETION IN RESPONSE TO GUANYLIN IN COLONIC EPITHELIA FROM NORMAL AND TRANSGENIC CYSTIC-FIBROSIS MICE [J].

CUTHBERT, AW ;

HICKMAN, ME ;

MACVINISH, LJ ;

EVANS, MJ ;

COLLEDGE, WH ;

RATCLIFF, R ;

SEALE, PW ;

HUMPHREY, PPA .

BRITISH JOURNAL OF PHARMACOLOGY, 1994, 112 (01) :31-36

[5] PRECURSOR STRUCTURE, EXPRESSION, AND TISSUE DISTRIBUTION OF HUMAN GUANYLIN [J].

DESAUVAGE, FJ ;

KESHAV, S ;

KUANG, WJ ;

GILLETT, N ;

HENZEL, W ;

GOEDDEL, DV .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (19) :9089-9093

[6] CONSENSUS SEQUENCE FOR PROCESSING OF PEPTIDE PRECURSORS AT MONOBASIC SITES [J].

DEVI, L .

FEBS LETTERS, 1991, 280 (02) :189-194

[7] HEAT-STABLE ENTEROTOXIN OF ESCHERICHIA-COLI - INVITRO EFFECTS ON GUANYLATE CYCLASE ACTIVITY, CYCLIC-GMP CONCENTRATION, AND ION-TRANSPORT IN SMALL-INTESTINE [J].

FIELD, M ;

GRAF, LH ;

LAIRD, WJ ;

SMITH, PL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1978, 75 (06) :2800-2804

[8]

FORSSMANN WG, 1995, GASTROINTESTINAL TRA, V77, P279

[9] GUANYLIN - A PEPTIDE REGULATOR OF EPITHELIAL TRANSPORT [J].

FORTE, LR ;

CURRIE, MG .

FASEB JOURNAL, 1995, 9 (08) :643-650

[10] RAPID PRODUCTION OF FULL-LENGTH CDNAS FROM RARE TRANSCRIPTS - AMPLIFICATION USING A SINGLE GENE-SPECIFIC OLIGONUCLEOTIDE PRIMER [J].

FROHMAN, MA ;

DUSH, MK ;

MARTIN, GR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (23) :8998-9002