RESONANCE ASSIGNMENT OF METHIONINE METHYL-GROUPS AND CHI(3) ANGULAR INFORMATION FROM LONG-RANGE PROTON-CARBON AND CARBON-CARBON J-CORRELATION IN A CALMODULIN PEPTIDE COMPLEX

被引:52
作者
BAX, A
DELAGLIO, F
GRZESIEK, S
VUISTER, GW
机构
[1] Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, 20892, MD
关键词
CALMODULIN; CARBON-CARBON J COUPLING; CARBON PROTON J COUPLING; HMBC; METHIONINE; PROTEIN NMR; 3D NMR; ISOTOPE LABELING;
D O I
10.1007/BF00398409
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Several simple 3D experiments are used to provide J correlations between methionine C-epsilon methyl carbons and either the (CH2)-H-gamma protons or C-beta and C-gamma. The intensity of the J correlations provides information on the size of the three-bond J couplings and thereby on the chi(3) torsion angle. In addition, a simple 3D version of the HMBC experiment provides a sensitive link between the (CH3)-H-epsilon methyl protons and C-gamma. The methods are demonstrated for a 20 kDa complex between calmodulin and a 26-residue peptide fragment of skeletal muscle myosin light chain kinase.
引用
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页码:787 / 797
页数:11
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