COCRYSTALS OF ESCHERICHIA-COLI TRP REPRESSOR BOUND TO AN ALTERNATIVE OPERATOR DNA-SEQUENCE

被引：6

作者：

CAREY, J ^{[1
]}

COMBATTI, N ^{[1
]}

LEWIS, DEA ^{[1
]}

LAWSON, CL ^{[1
]}

机构：

[1] BROOKHAVEN NATL LAB,DEPT BIOL,UPTON,NY 11973

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1993年 / 234卷 / 02期

关键词：

DNA-PROTEIN COCRYSTALLIZATION; BIASED RANDOM SEARCH; COOPERATIVITY; DNA SEQUENCE CONTEXT;

D O I：

10.1006/jmbi.1993.1602

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cocrystals of a 2:1 complex of trp repressor dimers with a DNA duplex containing a single, central operator half-site sequence are described. Crystals with different morphologies grew under diverse crystallization conditions within days to weeks by hanging-drop vapor-diffusion. Twinned rods split along their longitudinal cleft produce single crystals with space group C2 and unit cell dimensions a = 112.34 Å, b = 90.16 Å, c = 58.65 Å and β = 113.92°. The crystals diffract to 2.4 Å and are thus suitable for structural analysis by X-ray diffraction. Several heavy-atom derivative cocrystals have been obtained with iodouridine-substituted DNAs. © 1993 Academic Press Limited.

引用

页码：496 / 498

页数：3

共 12 条

[1]

CAREY J, 1989, J BIOL CHEM, V264, P1941

[2]

CAREY J, 1991, J BIOL CHEM, V266, P24509

[3] CONTROLLING BASAL EXPRESSION IN AN INDUCIBLE T7 EXPRESSION SYSTEM BY BLOCKING THE TARGET T7 PROMOTER WITH LAC REPRESSOR [J].