SYNTHESIS AND PURIFICATION OF RADIOACTIVE FATTY ACYLCARNITINES OF HIGH SPECIFIC ACTIVITY

被引:3
作者
SQUIRE, RST [1 ]
机构
[1] CLIN RES INST MONTREAL, INTERMEDIARY METAB LAB, MONTREAL H2W 1R7, QUEBEC, CANADA
基金
英国医学研究理事会;
关键词
D O I
10.1016/0003-2697(91)90363-X
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Micromole quantities of radiolabeled fatty acylcarnitines of high specific activity were synthesized by a simple one-step, one-pot acylation of [3H]carnitine in the presence of the respective fatty acid and acid chloride. The acylated product was isolated in high yield and high purity using liquid-liquid partitioning with isopropanol:hydrochloric acid:heptane (4:1:12 v/v) followed by butanol extraction. Product recovery was 91% for octanoyl[3H]carnitine and 90% for palmitoyl[3H]carnitine and contamination of both products with carnitine was less than 1%. The method is simple and fast, and results in high yields of pure fatty acylcarnitine in micromole scale. © 1991.
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页码:104 / 107
页数:4
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