THE EFFECT OF ALUMINUM COATING ON ELEMENTAL STANDARDS IN X-RAY-MICROANALYSIS

被引：16

作者：

HOPKINS, DM ^{[1
]}

JACKSON, AD ^{[1
]}

OATES, K ^{[1
]}

机构：

[1] UNIV ST ANDREWS,GATTY MARINE LABS,ST ANDREWS KY16 9ST,FIFE,SCOTLAND

来源：

JOURNAL OF ELECTRON MICROSCOPY TECHNIQUE | 1991年 / 18卷 / 02期

关键词：

QUANTITATIVE X-RAY MICROANALYSIS; COATING PARAMETERS; FROZEN HYDRATED SPECIMENS;

D O I：

10.1002/jemt.1060180213

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The standardisation of frozen hydrated bulk biological specimens using gelatin standards is described. The relationship between corrected elemental X-ray counts and ionic concentration was found to be linear, and minimum detectable limits for each element are stated. Variations in uncorrected standard curves were found to be due to changes in aluminium coating thickness. There was an inverse relationship between coating thickness and elemental X-ray counts. The factors causing this are discussed. To avoid errors arising from inconsistent aluminium thickness, experimental material should only be compared with standards of similar aluminium net counts. This can be achieved most easily by mounting and analysing specimen and standard together.

引用

页码：176 / 182

页数：7

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