HYGROMYCIN RESISTANCE GENE CASSETTES FOR VECTOR CONSTRUCTION AND SELECTION OF TRANSFORMED RICE PROTOPLASTS

被引：44

作者：

ZHENG, ZW ^{[1
]}

HAYASHIMOTO, A ^{[1
]}

LI, ZJ ^{[1
]}

MURAI, N ^{[1
]}

机构：

[1] LOUISIANA STATE UNIV,COLL AGR,DEPT PLANT PATHOL & CROP PHYSIOL,BATON ROUGE,LA 70803

来源：

PLANT PHYSIOLOGY | 1991年 / 97卷 / 02期

关键词：

D O I：

10.1104/pp.97.2.832

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Hygromycin resistance gene cassettes were designed to facilitate vector construction for plant transformation. Unique EcoRI, Psi, and SacII sites in the coding sequence of a hygromycin B phosphotransferase gene (hph) from Escherichia coli were eliminated. The mutated hph genes were used to form gene cassettes flanked by EcoRI-SacII-KpnI-HindIII sites. Hygromycin resistance of wild-type and mutated hph genes was indistinguishable in E. coli and rice protoplast growth assay.

引用

页码：832 / 835

页数：4

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