QUANTITATIVE ASSESSMENT OF HEPATITIS-B VIRUS-DNA IN CHRONIC HEPATITIS-B - COMPARISON OF 2 SOLUTION HYBRIDIZATION ASSAYS

被引:18
作者
JANSSEN, HLA
SCHOENMAKERWEBER, YAM
KRUINING, H
SCHALM, SW
HEIJTINK, RA
机构
[1] ERASMUS UNIV ROTTERDAM, DEPT VIROL, DR MOLEWATERPLEIN 50, 3015 GD ROTTERDAM, NETHERLANDS
[2] UNIV HOSP DIJKZIGT, DEPT INTERNAL MED 2, ROTTERDAM, NETHERLANDS
关键词
CHRONIC HEPATITIS-B; HBV-DNA; AFFIPROBE ASSAY; ABBOTT HBV-DNA ASSAY;
D O I
10.1002/jmv.1890400408
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We compared two solution hybridization assays, the AffiProbe assay (Orion Corporation) and the Abbott HBV-DNA assay (Abbott), for quantitative measurement of hepatitis B virus (HBV) DNA in serum samples obtained from chronic hepatitis B surface antigen (HBsAg) carriers. Forty transversally collected (group 1) and 83 serially collected (group 2) serum samples from chronic hepatitis B patients were tested with both assays. The serial serum samples were obtained from 6 patients who underwent a-interferon therapy with different outcomes (nonresponse, hepatitis B e antigen [HBeAg] seroconversion, HBeAg and HBsAg seroconversion). In group 1 a good correlation (r = 0.91; P < 0.001) was found between the HBV-DNA results of the two assays. Two samples (5%) were HBV-DNA positive according to the Abbott but negative according to the AffiProbe assay; for all other samples the HBV-DNA status corresponded. In group 2 the assays gave colinear HBV-DNA results during follow-up of 5 of the 6 patients (correlation for the total group: r = 0.90; P < 0.001). Nevertheless, in both groups the AffiProbe assay yielded about 5-10 times higher HBV-DNA levels than the Abbott HBV-DNA assay (P < 0.001). These discordant results were most probably due to standardization differences of the positive control samples of the two test systems. This observation underlines the need for international standardization of HBV-DNA and uniform reference panels. (C) 1993 Wiley-Liss, Inc.
引用
收藏
页码:307 / 312
页数:6
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