MOLECULAR-CLONING OF THE COMMON CARP (CYPRINUS-CARPIO) RHODOPSIN CDNA

被引：10

作者：

TSAI, HJ ^{[1
]}

SHIH, SR ^{[1
]}

KUO, CM ^{[1
]}

LI, LK ^{[1
]}

机构：

[1] COLUMBIA UNIV,DEPT OPHTHALMOL RES HLTH SCI,NEW YORK,NY

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1994年 / 109卷 / 01期

关键词：

CARP; PCR SCREENING; PHOTORECEPTOR; RETINAL CDNA LIBRARY; RHODOPSIN; SIGNAL TRANSDUCTION; TRANSMEMBRANE DOMAIN; VISUAL PIGMENT;

D O I：

10.1016/0305-0491(94)90144-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A recombinant phage clone containing a 1584 nucleotides rhodopsin cDNA was screened from a carp retinal cDNA library. The inserted DNA consisting of a single open reading frame of 1062 nucleotides at positions 72 and 1133 encodes a 354 amino acid polypeptide. The deduced amino acid sequence of carp rhodopsin showed 95.7, 85.5 and 74.4% identity with that of goldfish, sand goby and lamprey, respectively. The sites of palmitoylation, glycosylation, disulfide bond formation and Schiff base formation in the putative rhodopsin are all conserved.

引用

页码：81 / 88

页数：8

共 25 条

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