SYNTHESES OF 15-ALPHA-CARBOXYMETHYLTESTOSTERONE AND 15-BETA-CARBOXYMETHYLTESTOSTERONE BOVINE SERUM-ALBUMIN CONJUGATES - CHARACTERISTICS OF THE ANTISERA TO TESTOSTERONE

被引：15

作者：

MIYAKE, Y

KUBO, Y

IWABUCHI, S

KOJIMA, M

机构：

来源：

STEROIDS | 1982年 / 40卷 / 03期

关键词：

D O I：

10.1016/0039-128X(82)90037-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Syntheses of 15.alpha.- and 15.beta.-carboxymethyltestosterone (15.alpha.- and 15.beta.-CMT) were investigated to prepare testosterone-bovine serum albumin conjugates for radioimmunoassays of testosterone. A mixture of 15.alpha.- and 15.beta.-bis(ethoxycarbonyl)methyl-3.beta.-hydroxy-5-androstein-17-one obtained by a reaction of 3.beta.-hydroxy-5,15-androstadien-17-one and sodium diethyl malonate was oxidized to afford a mixture of 15.alpha.- and 15.beta.-bis(ethoxycarbonyl)methyl-4-androstene-3,17-dione. After the separation by silica gel chromatography, each epimer obtained was hydrolyzed by acid, followed by decarboxylation, and selective reduction of the 17-ketone to give 15.alpha.- and 15.beta.-CMT. The antisera, generated in rabbits by immunization with the bovine serum albumin conjugates of 15.alpha.- and 15.beta.-CMT, respectively, exhibited high specificity for testosterone.

引用

页码：245 / 259

页数：15

共 27 条

[1] SOLID-PHASE RADIOIMMUNOASSAY OF ESTRADIOL-17BETA [J].