EFFECT OF CHRONIC ADMINISTRATION OF ANTIDEPRESSANTS ON ALPHA-2-ADRENOCEPTORS IN THE LOCUS-CERULEUS AND ITS PROJECTION FIELDS IN RAT-BRAIN DETERMINED BY QUANTITATIVE AUTORADIOGRAPHY

The density of alpha2-adrenoceptors, using H-3-idazoxan as the radioligand, was determined by quantitative autoradiography in the locus coeruleus and in 13 noradrenergic projection fields following chronic administration of drugs acting on noradrenergic and/or serotonergic neurons. Protriptyline, an inhibitor of the uptake of norepinephrine, and mianserin, an alpha2-adrenoceptor antagonist, reduced the binding of H-3-idazoxan only in the locus coeruleus. Phenelzine, an inhibitor of both type A and type B monoamine oxidase (MAO), reduced the binding of H-3-idazoxan in the locus coeruleus and in several areas with noradrenergic innervation from tegmental cell bodies. Clorgyline, a selective inhibitor of type A MAO, had no effect. Of the two selective inhibitors of serotonin uptake, citalopram caused a modest increase in binding only in one terminal field area, whereas sertraline had no effect. Although these antidepressants did not produce consistent effects on alpha2-adrenoceptors, protriptyline, mianserin, and phenelzine were similar in that they all decreased the binding of H-3-idazoxan in the locus coeruleus without widely affecting its binding in the coerulean terminal fields. Deprenyl, a selective inhibitor of type B MAO, the only drug in this study without proven antidepressant efficacy, differed from all other drugs in that it decreased the binding of H-3-idazoxan both in the locus coeruleus as well as in most terminal fields with primarily coerulean noradrenergic innervation.