PROTEINASES IN TRICHOMONAS-VAGINALIS AND TRITRICHOMONAS-MOBILENSIS ARE NOT EXCLUSIVELY OF CYSTEINE TYPE

被引：25

作者：

BOZNER, P ^{[1
]}

DEMES, P ^{[1
]}

机构：

[1] COMENIUS UNIV,INST IMMUNOL,CS-81108 BRATISLAVA,CZECHOSLOVAKIA

来源：

PARASITOLOGY | 1991年 / 102卷

关键词：

TRICHOMONAS-VAGINALIS; TRITRICHOMONAS-MOBILENSIS; PROTEINASES; METALLO-PROTEINASES;

D O I：

10.1017/S0031182000060418

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

High molecular weight proteinases of Trichomonas vaginalis (with apparent M(r) values 142 and > 220 kDa) and Tritrichomonas mobilensis (M(r) 67, 86, 104 and 120 kDa), optimally active at pH 8, were analysed in gelatin-containing polyacrylamide gels. All of these proteinases were resistant to serine-, aspartic- as well as cysteine proteinase inhibitors. Both proteolytic bands in T. vaginalis and two proteinases in T. mobilensis (67 and 104 kDa) were inhibited by EDTA and EGTA suggesting that they belong to the metallo-proteinase class. The 67 kDa proteinase of T. mobilensis was inhibited also by o-phenanthroline. The other two bands of T. mobilensis (86, 120 kDa) were not classified to any proteinase group since they appeared to be resistant to the chelating agents tested in this study.

引用

页码：113 / 115

页数：3

共 8 条

[1] SPECIFIC PARASITISM OF PURIFIED VAGINAL EPITHELIAL-CELLS BY TRICHOMONAS-VAGINALIS
ALDERETE, JF
DEMES, P
GOMBOSOVA, A
VALENT, M
FABUSOVA, M
JANOSKA, A
STEFANOVIC, J
ARROYO, R
[J]. INFECTION AND IMMUNITY, 1988, 56 (10) : 2558 - 2562
[2] PROTEOLYTIC ACTIVITY IN TRITRICHOMONAS-MOBILENSIS
BOZNER, P
DEMES, P
STEFANOVIC, J
[J]. PARASITOLOGY, 1990, 101 : 57 - 60
[3] COOMBS GH, 1983, PARASITOLOGY, V86, P1
[4] THE ESTABLISHMENT OF VARIOUS TRICHOMONADS OF ANIMALS AND MAN IN AXENIC CULTURES
DIAMOND, LS
[J]. JOURNAL OF PARASITOLOGY, 1957, 43 (04) : 488 - 490
[5] THE USE OF A HIGHLY SENSITIVE ELECTROPHORETIC METHOD TO COMPARE THE PROTEINASES OF TRICHOMONADS
LOCKWOOD, BC
NORTH, MJ
SCOTT, KI
BREMNER, AF
COOMBS, GH
[J]. MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1987, 24 (01) : 89 - 95
[6] TRICHOMONAS-VAGINALIS, TRITRICHOMONAS-FOETUS, AND TRICHOMITUS-BATRACHORUM - COMPARATIVE PROTEOLYTIC ACTIVITY
LOCKWOOD, BC
NORTH, MJ
COOMBS, GH
[J]. EXPERIMENTAL PARASITOLOGY, 1984, 58 (03) : 245 - 253
[7] ANALYSIS OF THE PROTEINASES OF REPRESENTATIVE TRICHOMONAS-VAGINALIS ISOLATES
NEALE, KA
ALDERETE, JF
[J]. INFECTION AND IMMUNITY, 1990, 58 (01) : 157 - 162
[8] THE SPECIFICITY OF TRICHOMONAD CYSTEINE PROTEINASES ANALYZED USING FLUOROGENIC SUBSTRATES AND SPECIFIC INHIBITORS
NORTH, MJ
ROBERTSON, CD
COOMBS, GH
[J]. MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1990, 39 (02) : 183 - 194

← 1 →