EVALUATION OF THE AMOUNT OF ACYL-COA ELONGASES IN LEEK (ALLIUM-PORRUM L) LEAVES

被引：6

作者：

BESSOULE, JJ

CREACH, A

LESSIRE, R

CASSAGNE, C

机构：

[1] Institut de Biochimie Cellulaire et Neurochimie, CNRS, Université de Bordeaux, Bordeaux

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1117卷 / 01期

关键词：

LIPID; ACYL-COA; VERY LONG CHAIN FATTY ACID (VLCFA); ANTIBODY; ELONGATING SYSTEM; ICOSANOYL-COA SYNTHASE; (ALLIUM-PORRUM);

D O I：

10.1016/0304-4165(92)90165-Q

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Polyclonal antibodies have been raised against the acyl-CoA elongase purified from leek epidermal cells. The antibodies recognize the fractions containing the elongating activity after DEAE or Ultrogel chromatography and their response with the other fractions is very low. The immune complex is immunoprecipitable with Protein A-Sepharose. 1% of the solubilized proteins from leek epidermis microsomes are immunoprecipitated. The immunoprecipitate contains an elongating activity which is 86 +/- 20-times that of the unbound fraction.

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页码：78 / 82

页数：5

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