PROCESS MONITORING BY FLOW-INJECTION IMMUNOASSAY - EVALUATION OF A SEQUENTIAL COMPETITIVE-BINDING ASSAY

被引：44

作者：

NILSSON, M

HAKANSON, H

MATTIASSON, B

机构：

[1] Department of Biotechnology, Chemical Centre, Lund University, S-221 00 Lund

来源：

JOURNAL OF CHROMATOGRAPHY | 1992年 / 597卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(92)80135-H

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new variation on the theme of flow-injection binding assays is presented, namely the sequential competitive binding assay, in which the sample containing the native antigen is first introduced into the flow system followed by a pulse of labelled antigen. The flow-injection binding assay was used in monitoring the effluent from column chromatographic separation of proteins. By applying a computer-based evaluation system, concentrations are automatically read and compensation is made for denaturation in the affinity sorbent.

引用

页码：383 / 389

页数：7

共 9 条

[1]

AVRAMEAS S, 1978, SCAND J IMMUNOL S7, V8, P7

[2] CHEMICAL COUPLING OF PEPTIDES AND PROTEINS TO POLYSACCHARIDES BY MEANS OF CYANOGEN HALIDES [J].