SITE-DIRECTED MUTAGENESIS REVEALS TRANSITION-STATE STABILIZATION AS A GENERAL CATALYTIC MECHANISM FOR AMINOACYL-TRANSFER RNA-SYNTHETASES

被引：20

作者：

BORGFORD, TJ ^{[1
]}

GRAY, TE ^{[1
]}

BRAND, NJ ^{[1
]}

FERSHT, AR ^{[1
]}

机构：

[1] UNIV LONDON IMPERIAL COLL SCI & TECHNOL,DEPT CHEM,LONDON SW7 2AY,ENGLAND

来源：

BIOCHEMISTRY | 1987年 / 26卷 / 23期

关键词：

D O I：

10.1021/bi00397a008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

引用

页码：7246 / 7250

页数：5

共 13 条

[1] THE VALYL-TRANSFER RNA-SYNTHETASE FROM BACILLUS-STEAROTHERMOPHILUS HAS CONSIDERABLE SEQUENCE HOMOLOGY WITH THE ISOLEUCYL-TRANSFER RNA-SYNTHETASE FROM ESCHERICHIA-COLI [J].

BORGFORD, TJ ;

BRAND, NJ ;

GRAY, TE ;

FERSHT, AR .

BIOCHEMISTRY, 1987, 26 (09) :2480-2486

[2] MOLECULAR-CLONING OF THE GENE ENCODING THE VALYL-TRANSFER-RNA SYNTHETASE FROM BACILLUS-STEAROTHERMOPHILUS [J].

BRAND, NJ ;

FERSHT, AR .

GENE, 1986, 44 (01) :139-142

[3] CRYSTAL-STRUCTURE OF A DELETION MUTANT OF A TYROSYL-TRANSFER RNA-SYNTHETASE COMPLEXED WITH TYROSINE [J].

BRICK, P ;

BLOW, DM .

JOURNAL OF MOLECULAR BIOLOGY, 1987, 194 (02) :287-297

[4] LIGAND-BINDING AND ENZYMIC CATALYSIS COUPLED THROUGH SUBUNITS IN TYROSYL-TRANSFER-RNA SYNTHETASE [J].

FERSHT, AR ;

MULVEY, RS ;

KOCH, GLE .

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[5] BINDING-ENERGY AND CATALYSIS - A LESSON FROM PROTEIN ENGINEERING OF THE TYROSYL-TRANSFER RNA-SYNTHETASE [J].

FERSHT, AR ;

LEATHERBARROW, RJ ;

WELLS, TNC .

TRENDS IN BIOCHEMICAL SCIENCES, 1986, 11 (08) :321-325

[6] TRANSITION-STATE STABILIZATION IN THE MECHANISM OF TYROSYL-TRANSFER RNA-SYNTHETASE REVEALED BY PROTEIN ENGINEERING [J].

LEATHERBARROW, RJ ;

FERSHT, AR ;

WINTER, G .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (23) :7840-7844

[7] LIGAND-BINDING STOICHIOMETRIES, SUBUNIT STRUCTURE, AND SLOW TRANSITIONS IN AMINOACYL-TRANSFER-RNA SYNTHETASES [J].

MULVEY, RS ;

FERSHT, AR .

BIOCHEMISTRY, 1977, 16 (18) :4005-4013

[8] CLONING IN SINGLE-STRANDED BACTERIOPHAGE AS AN AID TO RAPID DNA SEQUENCING [J].

SANGER, F ;

COULSON, AR ;

BARRELL, BG ;

SMITH, AJH ;

ROE, BA .

JOURNAL OF MOLECULAR BIOLOGY, 1980, 143 (02) :161-178

[9] AMINOACYL TRANSFER RNA-SYNTHETASES - GENERAL FEATURES AND RECOGNITION OF TRANSFER-RNAS [J].

SCHIMMEL, PR ;

SOLL, D .

ANNUAL REVIEW OF BIOCHEMISTRY, 1979, 48 :601-648

[10] FREE-ENERGY OF HYDROLYSIS OF TYROSYL ADENYLATE AND ITS BINDING TO WILD-TYPE AND ENGINEERED MUTANT TYROSYL-TRANSFER RNA-SYNTHETASES [J].

WELLS, TNC ;

HO, CK ;

FERSHT, AR .

BIOCHEMISTRY, 1986, 25 (21) :6603-6608

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