CRYSTAL-STRUCTURE OF A YEAST TBP TATA-BOX COMPLEX

被引：961

作者：

KIM, YC

GEIGER, JH

HAHN, S

SIGLER, PB

机构：

[1] YALE UNIV,DEPT MOLEC BIOPHYS & BIOCHEM,NEW HAVEN,CT 06510

[2] YALE UNIV,HOWARD HUGHES MED INST,NEW HAVEN,CT 06510

[3] FRED HUTCHINSON CANC RES CTR,SEATTLE,WA 98104

来源：

NATURE | 1993年 / 365卷 / 6446期

关键词：

D O I：

10.1038/365512a0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The 2.5 angstrom crystal structure of a TATA-box complex with yeast TBP shows that the eight base pairs of the TATA box bind to the concave surface of TBP by bending towards the major groove with unprecedented severity. This produces a wide open, underwound, shallow minor groove which forms a primarily hydrophobic interface with the entire under-surface of the TBP saddle. The severe bend and a positive writhe radically alter the trajectory of the flanking B-form DNA.

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页码：512 / 520

页数：9

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