PRESSURE STABILIZATION OF PROTEINS FROM EXTREME THERMOPHILES

被引:90
作者
HEI, DJ
CLARK, DS
机构
关键词
D O I
10.1128/AEM.60.3.932-939.1994
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
We describe the stabilization by pressure of enzymes, including a hydrogenase from Methanococcus jannaschii, an extremely thermophilic deep-sea methanogen. This is the first published report of proteins from thermophiles being stabilized by pressure. Inactivation studies of partially purified hydrogenases from an extreme thermophile (Methanococcus igneus), a moderate thermophile (Methanococcus thermolithotrophicus), and a mesophile (Methanococcus maripaludis), all from shallow marine sites, show that pressure stabilization is not unique to enzymes isolated from high-pressure environments. These studies suggest that pressure stabilization of an enzyme may be related to its thermophilicity. Further experiments comparing the effects of increased pressure on the stability of alpha-glucosidases from the hyperthermophile Pyrococcus furiosus and Saccharomyces cerevisiae support this possibility. We hale also examined pressure effects on several highly homologous glyceraldehyde-3-phosphate dehydrogenases from mesophilic and thermophilic sources and a rubredoxin from P. furiosus. The results suggest that hydrophobic interactions, which have been implicated in the stabilization of many thermophilic proteins, contribute to the pressure stabilization of enzymes from thermophiles.
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页码:932 / 939
页数:8
相关论文
共 41 条
[1]  
BLAKE P, 1992, BIOCHEMISTRY, V30
[2]   METHANOCOCCUS-IGNEUS SP-NOV, A NOVEL HYPERTHERMOPHILIC METHANOGEN FROM A SHALLOW SUBMARINE HYDROTHERMAL SYSTEM [J].
BURGGRAF, S ;
FRICKE, H ;
NEUNER, A ;
KRISTJANSSON, J ;
ROUVIER, P ;
MANDELCO, L ;
WOESE, CR ;
STETTER, KO .
SYSTEMATIC AND APPLIED MICROBIOLOGY, 1990, 13 (03) :263-269
[3]  
Constantino HR, 1990, J BACTERIOL, V172, P3654, DOI 10.1128/JB.172.7.3654-3660.1990
[4]  
CREIGHTON TE, 1990, BIOCHEM J, V270, P1
[5]   AMINO-ACID SEQUENCE OF GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE FROM LOBSTER MUSCLE [J].
DAVIDSON, BE ;
SAJGO, M ;
NOLLER, HF ;
HARRIS, JI .
NATURE, 1967, 216 (5121) :1181-&
[6]   Application of the theory of absolute reaction rates to bacterial luminescence [J].
Eyring, H ;
Magee, JL .
JOURNAL OF CELLULAR AND COMPARATIVE PHYSIOLOGY, 1942, 20 (02) :169-177
[7]   PYROCOCCUS-FURIOSUS SP-NOV REPRESENTS A NOVEL GENUS OF MARINE HETEROTROPHIC ARCHAEBACTERIA GROWING OPTIMALLY AT 100-DEGREES C [J].
FIALA, G ;
STETTER, KO .
ARCHIVES OF MICROBIOLOGY, 1986, 145 (01) :56-61
[8]  
HAMANN SD, 1980, REV PHYS CHEM JPN, V50, P147
[9]   GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE FROM PIG MUSCLE [J].
HARRIS, JI ;
PERHAM, PN .
NATURE, 1968, 219 (5158) :1025-&
[10]  
Harrison J., 1976, P1