ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR RAT AND HUMAN LUTEINIZING-HORMONE

被引:8
作者
LEIVA, LA [1 ]
DELALASTRA, M [1 ]
机构
[1] CATHOLIC UNIV CHILE,FAC CIENCIAS BIOL,INST CHILENO MED REPROD,SANTIAGO,CHILE
关键词
LUTEINIZING HORMONE; ELISA; RAT LH; HUMAN LH;
D O I
10.1159/000126290
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
A sensitive and specific enzyme-linked immunosorbent assay (ELISA) suitable for measuring rat and human luteinizing hormone (LH) is described. The LH-ELISA used anti-bLH beta subunit monoclonal antibody-coated plates, an antiserum against rLH or hLH and an antibody against rabbit IgG labelled with peroxidase. Using rLH-RP-3 or hLH WHO IRP 68/40 as standard diluted in assay buffer, the LH-ELISA had a sensitivity of 50 pg/ml and 0.78 mIU/ml, respectively. The LH-ELISA allowed accurate determination of LH in buffer, cell culture media, anterior pituitary extracts and sera and was highly specific for rat and human LH. The use of this ELISA offers improvements in convenience, economy, sensitivity and safety over comparable radioimmunoassay procedures.
引用
收藏
页码:660 / 665
页数:6
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