PREPARATION OF ACTINOMYCETE DNA FOR PULSED-FIELD GEL-ELECTROPHORESIS

被引：7

作者：

BEYAZOVA, ML

BRODSKY, BC

SHEARER, MC

HORAN, AC

机构：

[1] Schering-Plough Research Institute, Kenilworth, NJ 07033-0539

来源：

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY | 1995年 / 45卷 / 04期

关键词：

D O I：

10.1099/00207713-45-4-852

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A procedure for preparing actinomycete DNA samples for pulsed-field gel electrophoresis is described. When this method was used, DNAs from strains belonging to the genera Streptomyces, Kutzneria, Dactylosporangium, Microtetraspora, Actinoplanes, Saccharothrix, and Micromonospora were successfully restriction digested with infrequently cutting enzymes, such as DraI and AseI.

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页码：852 / 854

页数：3

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