CHLOROPLAST DNA DELETIONS ASSOCIATED WITH WHEAT PLANTS REGENERATED FROM POLLEN - POSSIBLE BASIS FOR MATERNAL INHERITANCE OF CHLOROPLASTS

被引：160

作者：

DAY, A ^{[1
]}

ELLIS, THN ^{[1
]}

机构：

[1] JOHN INNES INST, NORWICH NR4 7UH, NORFOLK, ENGLAND

来源：

CELL | 1984年 / 39卷 / 02期

关键词：

D O I：

10.1016/0092-8674(84)90014-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Albinism in plants regenerated from pollen by anther culture may result from the same mechanisms as postulated to be responsible for maternal inheritance of chloroplasts. Consistent with this view, Southern blotting was used to show that large regions of the chloroplast genome (over 80% in 1 case) were deleted in the major ct[chloroplast]DNA molecules present in 8 albino wheat plants regenerated from separate pollen calluses. A wide variety of deleted ctDNA molecules was present in these plants. Most albino plants appeared to contain a heterogeneous population of ctDNA molecules. Albino plant 6 contained 1 major type of deleted ctDNA molecule that had a circular 39 kiloblase restriction map. The retention of only 2 Pst I (unaltered in size) fragments in albino plant 7 may delimit a region of ctDNA that contains a replication origin.

引用

页码：359 / 368

页数：10

共 58 条

[1] ON THE FORMATION OF SPONTANEOUS DELETIONS - THE IMPORTANCE OF SHORT SEQUENCE HOMOLOGIES IN THE GENERATION OF LARGE DELETIONS [J].