PROPOSAL FOR SEQUENCING DNA USING LIGATION OF HEXAMERS TO GENERATE SEQUENTIAL ELONGATION PRIMERS (SPEL-6)

被引：42

作者：

SZYBALSKI, W

机构：

[1] McArdle Laboratory for Cancer Research, University of Wisconsin, Madison

来源：

GENE | 1990年 / 90卷 / 01期

关键词：

'top-down' and 'bottom-up' strategies; 6-mer library; cosmids; directed priming; oligodeoxyribonucleotides; Recombinant DNA;

D O I：

10.1016/0378-1119(90)90458-4

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The efficiency of the directed-priming proposal of Studier [Proc. Natl. Acad. Sci. USA 86 (1989) 6917-1921] can be improved upon by employing a library of less than 4094 hexamers, allowing for the easy construction of all required primers using template-directed automated ligation. This 'top-down' sequencing procedure should be several times more efficient than the present 'bottom-up' procedures [see Watson, Science 248 (1990) 44-49]. © 1990.

引用

页码：177 / 178

页数：2