EFFICIENT SYNTHESIS OF OLIGORIBONUCLEOTIDE AND ITS PHOSPHOROTHIOATE ANALOG USING H-PHOSPHONATE APPROACH

被引：32

作者：

AGRAWAL, S

TANG, JY

机构：

[1] Worcester Foundation for Experimental Biology, Shrewsbury, MA 01545

来源：

TETRAHEDRON LETTERS | 1990年 / 31卷 / 52期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/S0040-4039(00)97293-9

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Efficient solid phase synthesis of oligoribonucleotide and its phosphorothioate analogue is described that utilizes the dimethoxytrityl (DMTr) for 5'-protection and t-butyldimethylsilyl (t-BDMS) group for 2'-protection of ribonucleoside monomers and the H-phosphonate coupling procedure. The synthetic cycles have been optimised to use only 8-10 fold excess of monomer at each coupling step, leading to an average coupling yield of 97%.

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收藏

页码：7541 / 7544

页数：4

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