SOFT-X-RAY SPECTROSCOPY OF METALLOPROTEINS USING FLUORESCENCE DETECTION

被引：34

作者：

CRAMER, SP

CHEN, J

GEORGE, SJ

VANELP, J

MOORE, J

TENSCH, O

COLARESI, J

YOCUM, M

MULLINS, OC

CHEN, CT

机构：

[1] UNIV CALIF BERKELEY,DIV ENERGY & ENVIRONM,BERKELEY,CA 94720

[2] CANBERRA IND,MERIDEN,CT 06450

[3] SCHLUMBERGER DOLL RES CTR,RIDGEFIELD,CT 06877

[4] AT&T BELL LABS,MURRAY HILL,NJ 07974

来源：

NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION A-ACCELERATORS SPECTROMETERS DETECTORS AND ASSOCIATED EQUIPMENT | 1992年 / 319卷 / 1-3期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

D O I：

10.1016/0168-9002(92)90567-N

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

Fluorescence detection methods have been developed for measuring the L2,3 X-ray absorption spectra of first transition series metalloproteins. Samples are prepared as thin films on silicon supports, and mounted on a liquid helium cooled cold finger in a UHV chamber. A windowless Ge array detector discriminates metal L fluorescence from oxygen K(alpha) background. The high resolution, strong sensitivity to chemical environment and amenability to quantitative spectral shape analysis indicate that L-edges of the first transition series metals are a useful probe for bioinorganic studies.

引用

页码：285 / 289

页数：5

共 16 条

[1] PERFORMANCE OF THE DRAGON SOFT-X-RAY BEAMLINE [J].