GENE-TRANSFER INTO CIRCULATING PRIMORDIAL GERM CELLS OF QUAIL EMBRYOS

被引：5

作者：

ONO, T

MUTO, S

MATSUMOTO, T

MOCHII, M

EGUCHI, G

机构：

[1] Faculty of Agriculture, Shinshu University

[2] Kissei Pharmaceutical Co., Ltd.

[3] Division of Morphogenesis, National Institute for Basic Biology

来源：

EXPERIMENTAL ANIMALS | 1995年 / 44卷 / 04期

关键词：

LACZ; LIPOFECTION; PMIWZ; PRIMORDIAL GERM CELLS; QUAIL;

D O I：

10.1538/expanim.44.275

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

During early stages in avian embryogenesis primordial germ cells (PGCs) show a unique migration pathway toward the gonadal anlage through the circulation. In the present study, liposomes consisting of plasmid DNA (pMiwZ; containing lacZ as a reporter) and Lipofectin were injected into the marginal veins of quail embryos during the stages PGCs were circulating in the blood vessels. The lacZ expression was then histochemically detected in the gonads at later embryonic stages, indicating the expression of the injected DNA in PGCs.

引用

页码：275 / 278

页数：4

共 18 条

[1]

Ando Y., Fujimoto T., Ultrastructural evidence that chick primordial germ cells leave the blood-vascular system prior to migrating to the gonadal anlagen, Develop. Growth & Differ, 25, pp. 345-352, (1983)

[2]

Bosselman R.A., Hsu R-Y., Boggs T., Hu S., Bruszewski J., Ou S., Kozar L., Martin F., Green C., Jacobsen F., Nicolson M., Schults J.A., Semon K.M., Rishell W., Stewart R.G., Germ line transmission of exogenous genes in the chicken, Science, 243, pp. 533-535, (1989)

[3]

Brazolot C.L., Petitte J.N., Etches R.J., Verrinder Gibbins A.M., Efficient translocation of chicken cells by Lipofectin, and introduction of transfected blastodermal cells into the embryo, Mol. Reprod. Devel, 30, pp. 304-312, (1991)

[4]

Cheng K.M., Kimura M., Mutations and major variants in Japanese quail, pp. 333-362, (1990)

[5]

Eyal-Giladi H., Ginsburg M., Fabarov A.A., Avian primordial cells are of epiblastic origin, J. Embryol. Exp. Morphol, 65, pp. 139-147, (1981)

[6]

Fujimoto T., Ukeshima A., Kiyofuji R., The origin, migration and morphology of the primordial germ cells in the chick embryo, Anat. Rec, 185, pp. 139-154, (1976)

[7]

Ginsburg M., Eyal-Giladi H., Temporal and spatial aspects of the gradual migration of primordial germ cells from the epiblast into the germinal crescent in the avian embryo, J. Embryol. Exp. Morphol, 95, pp. 53-71, (1986)

[8]

Hamburger V., Hamilton H.L., A series of normal stages in the development of the chick embryo, J. Morphol, 88, pp. 49-92, (1951)

[9]

Kuwana T., Migration of avian primordial germ cells toward the gonadal anläge, Develop. Growth & Differ, 35, pp. 237-243, (1993)

[10]

Love J., Gribbin C., Mather C., Sang H., Transgenic birds by DNA microinjection, Bio/Technology, 12, pp. 60-63, (1994)

← 1 2 →