LIPOLYTIC ENZYMES OF THE HUMAN-PANCREAS .1. A HIGHLY SENSITIVE, SPECIFIC, AND SIMPLE MEASUREMENT OF CHOLESTEROL ESTER HYDROLASE ACTIVITY

被引：11

作者：

MEYERBERTENRATH, JG ^{[1
]}

机构：

[1] STADTKRANKENHAUS HANAU, ZENTRALLAB, D-6450 HANAU, FED REP GER

来源：

ENZYME | 1982年 / 28卷 / 04期

关键词：

D O I：

10.1159/000459120

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cholesterol ester hydrolase (cholesterol esterase) acts on cholesterol esters and rs of fluorescein. Diesters and monoesters of this dye show neither color nor fluorescence, so that these compounds may be used as chromogenic or fluorogenic substrates. Splitting off the ester bonds immediately causes the return of the original properties of the fluorescein. Its dilaurate is a specific substrate of cholesterol esterase, allowing the measurement of activities of this enzyme down to ranges of 0.01 U/l by a fluorometric assay.

引用

页码：336 / 342

页数：7

共 26 条

[1] PANCREATIC NONSPECIFIC LIPASE, AN ENZYME HIGHLY SPECIFIC FOR MICELLES [J].

ALBRO, PW ;

LATIMER, AD .

BIOCHEMISTRY, 1974, 13 (07) :1431-1439

[2]

COUTTS JRT, 1967, BIOCHEM J, V103, pP55

[3]

GALLO LL, 1978, J LIPID RES, V19, P913

[4]

HERNANDEZ HH, 1957, J BIOL CHEM, V228, P447

[5] PANCREATIC-JUICE CHOLESTEROL ESTERASE - STUDIES ON MOLECULAR WEIGHT AND BILE SALT-INDUCED POLYMERIZATION [J].

HYUN, J ;

VAHOUNY, GV ;

TREADWEL.CR .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1972, 152 (01) :233-&

[6]

HYUN J, 1969, J BIOL CHEM, V244, P1937

[7]

KAFFARNIK H, 1971, Verhandlungen der Deutschen Gesellschaft fuer Innere Medizin, V77, P524

[8] METHOD AND CLINICAL IMPORTANCE OF A NEW PANCREATIC LIPASE ASSAY WITH DI-DODECANOIC ACID ESTER OF FLUORESCEINE [J].

KAFFARNIK, H ;

MEYERBER.JG .

KLINISCHE WOCHENSCHRIFT, 1969, 47 (04) :221-+

[9]

Kaffarnik H, 1968, Verh Dtsch Ges Inn Med, V74, P237

[10]

Kaffarnik H, 1969, Verh Dtsch Ges Inn Med, V75, P635

← 1 2 3 →